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Updated: Jun 25, 2025
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
Published on: May 23, 2025
CRISPR-Cas and CRISPR-based screening system for precise gene editing and targeted cancer therapy
Mingming Qin1,2, Chunhao Deng3, Liewei Wen4
1Reproductive Medical Center, Affiliated Foshan Maternity & Child Healthcare Hospital, Southern Medical University (Foshan Women and Children Hospital), Foshan, Guangdong, 528000, China.
Abstract:
Target cancer therapy has been developed for clinical cancer treatment based on the discovery of CRISPR (clustered regularly interspaced short palindromic repeat) -Cas system. This forefront and cutting-edge scientific technique improves the cancer research into molecular level and is currently widely utilized in genetic investigation and clinical precision cancer therapy. In this review, we summarized the genetic modification by CRISPR/Cas and CRISPR screening system, discussed key components for successful CRISPR screening, including Cas enzymes, guide RNA (gRNA) libraries, target cells or organs. Furthermore, we focused on the application for CAR-T cell therapy, drug target, drug screening, or drug selection in both ex vivo and in vivo with CRISPR screening system. In addition, we elucidated the advantages and potential obstacles of CRISPR system in precision clinical medicine and described the prospects for future genetic therapy.In summary, we provide a comprehensive and practical perspective on the development of CRISPR/Cas and CRISPR screening system for the treatment of cancer defects, aiming to further improve the precision and accuracy for clinical treatment and individualized gene therapy.
Insights
CRISPR-Cas systems enable precise genetic modification for cancer research and therapy. This review details CRISPR screening applications in CAR-T cell therapy and drug development for improved cancer treatment.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- CRISPR-Cas systems have revolutionized molecular biology and cancer research.
- These systems are crucial for genetic investigation and precision cancer therapy.
Purpose of the Study:
- To review CRISPR-Cas and CRISPR screening systems in cancer research.
- To discuss applications in CAR-T cell therapy, drug targeting, and screening.
- To explore CRISPR's advantages, obstacles, and future in precision medicine.
Main Methods:
- Summarizing genetic modification techniques using CRISPR/Cas.
- Analyzing key components for CRISPR screening (Cas enzymes, gRNA libraries, target cells).
- Reviewing applications in ex vivo and in vivo settings for CAR-T therapy and drug development.
Main Results:
- CRISPR screening facilitates CAR-T cell therapy optimization.
- It aids in identifying drug targets and screening potential therapeutics.
- CRISPR systems offer significant advantages for precision cancer medicine.
Conclusions:
- CRISPR/Cas and screening systems are vital tools for advancing cancer therapy.
- Further development promises enhanced precision and accuracy in individualized gene therapy.
- Overcoming obstacles will unlock the full potential of CRISPR in clinical settings.
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