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CmBr confers fruit bitterness under CPPU treatment in melon.

Mingyan Wang1, Naiyu Jiang1, Yuanchao Xu2

  • 1Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, Laboratory of Quality & Safety Risk Assessment for Fruit (Qingdao), Ministry of Agriculture and Rural Affairs, National Technology Centre for Whole Process Quality Control of FSEN Horticultural Products (Qingdao), College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China.

Plant Biotechnology Journal
|May 31, 2024
PubMed
Summary
This summary is machine-generated.

CPPU treatment can cause bitterness in melons due to cucurbitacin B. Researchers identified the CmBr gene as a key regulator, with CmRSM1 activating its expression, enabling the development of non-bitter melon varieties.

Keywords:
CPPUCmBrRSM1bitternesscucurbitacinmelon

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Area of Science:

  • Plant Biology
  • Biochemistry
  • Genetics

Background:

  • Abiotic factors like CPPU can induce bitterness in cucurbits, primarily through cucurbitacin B (CuB) in melons.
  • The molecular pathways governing CuB biosynthesis under such conditions are not fully understood.

Purpose of the Study:

  • To elucidate the genetic regulation of CPPU-induced CuB biosynthesis in melon.
  • To identify key genes and transcription factors involved in this pathway.
  • To explore breeding strategies for non-bitter melon varieties.

Main Methods:

  • Gene identification and expression analysis in melon fruit.
  • CRISPR/Cas9 gene editing to validate gene function.
  • Promoter analysis and electrophoretic mobility shift assays (EMSAs) to study transcription factor binding.
  • Development of introgression lines for breeding applications.

Main Results:

  • A set of genes involved in CPPU-induced CuB biosynthesis was identified, with CmBr identified as a major regulator.
  • CRISPR/Cas9 confirmed CmBr's essential role in CuB accumulation under CPPU treatment.
  • A MYB-related transcription factor, CmRSM1, was found to activate CmBr expression by binding to its promoter.
  • An introgression line with a mutated Cmbr gene successfully eliminated CPPU-induced bitterness.

Conclusions:

  • CmBr is a critical regulator of CuB biosynthesis in response to CPPU.
  • CmRSM1 acts as a positive regulator of CmBr, mediating the CPPU response.
  • The identified genetic mechanism provides a valuable target for breeding non-bitter melon varieties.
  • This research offers insights into secondary metabolite regulation under environmental stress.