Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Functional responses of 3 generalist predators to Thrips parvispinus (Thysanoptera: Thripidae): Amblydromalus limonicus and Amblyseius swirskii (Acari: Phytoseiidae), and Franklinothrips vespiformis (Thysanoptera: Aeolothripidae).

Journal of economic entomology·2026

Same author

Identification of distinct clinical phenotypes and their neurobiological signatures in stress-exposed individuals: A multimodal machine learning approach.

European psychiatry : the journal of the Association of European Psychiatrists·2026

Same author

Complete genome sequence of <i>Enterococcus faecium</i> strain AJ_C_05 with potential characteristics to break down carbohydrates, applicable to livestock industry.

Journal of animal science and technology·2026

Same author

IL-27-mediated hematopoietic dysregulation exacerbates disease severity in severe fever with thrombocytopenia syndrome virus infection.

Science translational medicine·2026

Same author

Effect of Agrobacterium tumefaciens on papaya whitefly Trialeurodes variabilis populations infesting papaya Carica papaya.

Scientific reports·2026

Same author

Two-dimensional GeTe nanosheets for psoriasis through modulation of macrophage activation and psoriatic inflammation.

Biomaterials science·2026

Same journal

Pathology-Targeted Nanoparticles Guided by Peptide Remodel the Periodontal Microenvironment for Periodontitis Therapy.

Advanced healthcare materials·2026

Same journal

Gellan Gum Fluid Gel System for Controlled-Delivery of Cytokine-Licensed MSC-EVs to Enhance Corneal Repair in Limbal Stem Cell Deficiency.

Advanced healthcare materials·2026

Same journal

Diabetic Wound Healing Enabled by a Tri-Functional MAX Phase via Cu Doping.

Advanced healthcare materials·2026

Same journal

Plasmonically Reinforced Self-Sufficient Nanozymes Dysregulating Redox Homeostasis for Augmented Cascade Catalytic Oncotherapy.

Advanced healthcare materials·2026

Same journal

Spontaneous Helical Alignment of Smooth Muscle Cells to Form a Medial Layer for Engineered Microvasculature.

Advanced healthcare materials·2026

Same journal

Injectable Antifouling Adhesive Hydrogel Enables Robust Neural Interfaces for Stable ECoG Recording.

Advanced healthcare materials·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 25, 2025

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

Published on: January 28, 2018

Reconfigurable Hanging Drop Microarray Platform for On-Demand Preparation and Analysis of Spheroid Array.

Hwisoo Kim¹, Bumsoo Kim², Soo Jee Kim¹

¹Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Advanced Healthcare Materials

|May 31, 2024

Summary

This summary is machine-generated.

This study introduces CLiCK-Spheroid, a novel 3D cell culture kit for high-throughput spheroid production and analysis. The platform enables controlled generation of diverse spheroid sizes and compositions for advanced cancer research.

Keywords:

contact‐based spheroid transfer gradient spheroid array hanging drop spheroids tumor microenvironment

More Related Videos

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Published on: July 5, 2019

Related Experiment Videos

Last Updated: Jun 25, 2025

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

A Paired Bead and Magnet Array for Molding Microwells with Variable Concave Geometries

Published on: January 28, 2018

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Lab-on-a-CD Platform for Generating Multicellular Three-dimensional Spheroids

Published on: November 7, 2019

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Published on: July 5, 2019

Area of Science:

Biotechnology
Cancer Research
3D Cell Culture

Background:

Growing demand for spheroid-based cancer research necessitates advanced platforms.
Tumor size and composition critically impact internal structure and function.
Need for scalable production of diverse spheroids.

Purpose of the Study:

Introduce CLiCK-Spheroid, a configurable and linkable 3D cell culture kit.
Facilitate high-throughput spheroid production and multiplexed analysis.
Enable generation of size- and composition-gradient spheroid arrays.

Main Methods:

Utilized a hanging drop microarray (HDMA) for homogeneous spheroid arrays.
Combined HDMA with gradient blocks for one-step size-gradient spheroid generation.
Employed a concave pillar microarray (CPMA) for spheroid compartmentalization and regrouping.

Main Results:

Demonstrated necrotic core formation correlated with spheroid size using size-gradient arrays.
Achieved high-throughput production of cell composition-gradient spheroid arrays.
Observed variations in morphology and vascularization based on cell composition.

Conclusions:

CLiCK-Spheroid offers a versatile platform for spheroid generation and analysis.
Enables scalable production of diverse spheroids for cancer research.
Facilitates multiplexed drug treatments and investigation of spheroid properties.