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Related Experiment Video

Updated: Jun 25, 2025

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
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Diving head-first into brain intravital microscopy.

Althea R Suthya1, Connie H Y Wong1, Joshua H Bourne1

  • 1Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Monash Medical Centre, Monash University, Clayton, VIC, Australia.

Frontiers in Immunology
|May 31, 2024
PubMed
Summary

Intravital microscopy (IVM) allows real-time observation of complex biological processes in living tissues, advancing our understanding of cell migration and function, especially in the brain.

Keywords:
brainimagingintravital microscopyneuroinflammationstroke

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Area of Science:

  • Neurobiology
  • Cellular Biology
  • Microscopy

Background:

  • Tissue microenvironments are complex and difficult to model ex vivo or in vitro.
  • Observing dynamic cellular activities in real-time in vivo is crucial for understanding physiological and pathological processes.
  • Intravital microscopy (IVM) has evolved significantly, enabling visualization in various tissue beds.

Purpose of the Study:

  • To review the application of rodent intravital microscopy (IVM) in neurobiology.
  • To discuss the capabilities and limitations of emerging IVM technologies for brain imaging.
  • To explore the use of these technologies in studying neuroinflammation.

Main Methods:

  • Review of scientific literature on intravital microscopy techniques.
  • Discussion of advancements in imaging technologies such as photoacoustic, two-photon, and three-photon microscopy.
  • Analysis of IVM applications in rodent models for neurobiological research.

Main Results:

  • Modern IVM techniques offer high-speed acquisition and enhanced resolution for in vivo imaging.
  • IVM has expanded the understanding of cell migration and function, particularly in challenging tissues like the brain.
  • Emerging technologies provide new capabilities for studying brain dynamics in health and disease.

Conclusions:

  • Intravital microscopy is a powerful tool for studying complex biological systems in vivo.
  • Advanced IVM techniques, including photoacoustic and multi-photon imaging, are revolutionizing neurobiology research.
  • IVM plays a critical role in investigating neuroinflammation and other neurological conditions.