Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

38.8K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
38.8K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

96.4K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
96.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ecosystem state and soil depth determine the microbial community response to warming in coastal marsh soils.

iScience·2026
Same author

Microbial diversity and community shifts in a petroleum reservoir under production: effects of water breakthrough and anthropogenic alterations.

Frontiers in microbiology·2026
Same author

Metabolic transitions along a moisture gradient in a poly-extreme high-altitude desert ecosystem within the Atacama Desert.

Environmental microbiome·2026
Same author

Cryptic sulfur cycling in the deep biosphere of ferruginous Lake Towuti, Indonesia.

Frontiers in microbiology·2025
Same author

Microbial ecology of subsurface granitic bedrock: a humid-arid site comparison in Chile.

ISME communications·2025
Same author

Hydrology masks warming effects on microbial communities in salt marsh soils.

FEMS microbiology ecology·2025
Same journal

Novel plasmid pCM3 harboring the <i>aph(3)</i> gene confers phosphorylation-driven streptomycin resistance in <i>Clavibacter michiganensis</i>.

mLife·2026
Same journal

SARS-CoV-2 enhances lysosomal exocytosis and deacidifies lysosomes to facilitate viral release.

mLife·2026
Same journal

Microbially driven organic carbon cycling at the land-sea interface: Advances and an integrated study framework.

mLife·2026
Same journal

The toxin-antitoxin complex Fic-1-AntF functions as a deAMPylase that regulates the activity of DNA gyrase.

mLife·2026
Same journal

Protists show high resilience and thrive under multiple chemical stressors.

mLife·2026
Same journal

Harnessing microbial power to degrade hydrocarbon-based plastics.

mLife·2026
See all related articles

Related Experiment Video

Updated: Jun 25, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.9K

A modified isooctane-based DNA extraction method from crude oil.

Armando Alibrandi1, Rolando di Primio2, Alexander Bartholomäus1

  • 1GFZ German Research Centre for Geoscience, Section Geomicrobiology Potsdam Germany.

Mlife
|May 31, 2024
PubMed
Summary
This summary is machine-generated.

A new method efficiently extracts microbial DNA from crude oil, enabling better understanding of reservoir microbes. This helps mitigate economic losses and safety risks associated with oil biodegradation and souring.

Keywords:
DNA extraction methodcrude oiloil reservoir microbiome

More Related Videos

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

20.7K
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
13:32

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

56.4K

Related Experiment Videos

Last Updated: Jun 25, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
12:33

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Published on: July 28, 2017

12.9K
Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
09:10

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Published on: June 24, 2016

20.7K
DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
13:32

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Published on: March 26, 2011

56.4K

Area of Science:

  • Microbiology
  • Petroleum Geochemistry
  • Biotechnology

Background:

  • Microbial activity in oil reservoirs significantly impacts petroleum composition, leading to economic losses and safety concerns through processes like biodegradation and souring.
  • Understanding reservoir microbial communities is vital for managing these impacts, but DNA extraction from complex crude oil matrices presents a significant challenge.

Purpose of the Study:

  • To develop and validate a novel, robust DNA extraction method suitable for diverse crude oil types, covering a wide range of American Petroleum Institute (API) gravity.
  • To optimize solvent, surfactant, and DNA extraction kit selection for maximizing DNA yield and microbial community data quality from oil samples.

Main Methods:

  • Investigated various solvents (e.g., isooctane) and surfactants (nonionic and ionic, including sodium dodecyl sulfate) for cell extraction from oils.
  • Evaluated three distinct DNA extraction kits, including the PowerSoil Pro Kit.
  • Applied the optimized method to aseptically collected oil reservoir samples with low microbial cell densities (10^1-10^3 cells/ml).

Main Results:

  • The optimal method combined isooctane solvent, sodium dodecyl sulfate (ionic surfactant), and the PowerSoil Pro Kit, yielding the highest DNA quantity and 16S rRNA gene sequencing reads.
  • The method successfully recovered microbial DNA from low-density samples, achieving an average of 41,431 (±8860) 16S rRNA sequencing reads per sample.
  • Indigenous thermophilic, halophilic, and anaerobic microbial taxa were consistently detected across all analyzed oil reservoir samples.

Conclusions:

  • The developed DNA extraction protocol is effective for analyzing microbial communities in crude oils across a broad API gravity range, overcoming previous limitations.
  • This advancement facilitates a deeper understanding of reservoir microbial ecology, crucial for mitigating detrimental processes like biodegradation and souring.
  • No correlation was observed between API gravity and DNA yield, indicating the method's broad applicability despite variations in oil density.