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Natural Compounds that Modulate Circadian Rhythms.
Tatsunosuke Tomita1, Renu Wadhwa1, Yoshiaki Onishi2
1AIST-INDIA DAILAB, Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial, Science & Technology (AIST), Central 5-41, 1-1-1 Higashi, Tsukuba, 305-8565, Japan.
Current Topics in Medicinal Chemistry
|May 31, 2024
Summary
Circadian rhythms, regulated by clock genes, impact health and disease. New cell-based assays help screen natural compounds for safe circadian rhythm modulation and therapeutic potential.
Area of Science:
- Chronobiology and molecular genetics.
- Investigating the fundamental biological processes of circadian rhythms.
Background:
- Circadian rhythms are 24-hour cycles conserved across species, regulated by clock genes controlling gene expression and physiological functions.
- Approximately 40% of protein-coding genes in mice exhibit 24-hour oscillations, highlighting their critical role in bodily functions.
- Disrupted circadian rhythms are linked to diseases like cancer and are exacerbated by modern lifestyles, affecting humoral, immunological, and neuronal pathways.
Purpose of the Study:
- To provide an overview of living cell-embedded real-time reporter gene assays for screening circadian rhythm-modulating compounds.
- To discuss the potential of natural compounds for circadian rhythm modulation validated by cell-based assays.
- To explore the therapeutic role of these compounds in disease treatment.
Main Methods:
- Overview of living cell-embedded real-time reporter gene assays.
- Screening of compounds for their ability to modulate circadian rhythmicity.
- Validation of natural compounds using cell-based assay systems.
Main Results:
- Development of real-time reporter gene assays for screening circadian rhythm modulators.
- Identification and validation of potential natural compounds with circadian rhythm-modulating effects.
- Demonstration of cell-based assay systems for reliable read-outs.
Conclusions:
- Living cell-based assays offer a reliable method for screening compounds that modulate circadian rhythm.
- Certain natural compounds show potential for therapeutic applications in diseases linked to circadian disruption.
- Further research and clinical investigations are warranted for safe and sustainable circadian rhythm modulation.
Keywords:
Cell-based assayCircadian rhythmLuciferaseMolecular clockNatural compounds.Real-time reporter gene assayMore Related Videos
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