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A simultaneous assay for T-cells and B-cells using Immunobeads.
American Journal of Clinical Pathology
|February 1, 1985
Summary
A new Quantigen assay accurately enumerates T- and B-lymphocytes using Immunobeads. This light microscope method correlates well with established techniques and flow cytometry, proving effective for chronic lymphocytic leukemia (B-CLL) cell analysis.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Accurate enumeration of T- and B-lymphocytes is crucial for diagnosing and monitoring various hematological malignancies and immune disorders.
- Existing methods for lymphocyte enumeration can be labor-intensive or require specialized equipment.
Purpose of the Study:
- To evaluate a new, commercially available light microscope assay (Quantigen) for simultaneous T- and B-lymphocyte enumeration.
- To compare the performance of the Quantigen assay against established methods and flow cytometry.
Main Methods:
- The Quantigen assay utilizes two color-coded Immunobeads for T-cell and B-cell labeling.
- Phagocytic cells are identified by their ingestion of both bead types.
- Comparisons were made with the E-rosette method for T-cells and FITC-labeling for B-cells.
- Flow cytometric analysis was also used for comparative evaluation.
Main Results:
- A high degree of correlation was observed between the Quantigen assay and existing methods (E-rosette and FITC-labeling).
- The Quantigen assay showed good agreement with flow cytometric analyses.
- Chronic lymphocytic leukemia (B-CLL) cells were identified as double-markers due to the T101 monoclonal antibody recognizing both T-cells and B-CLL cells.
Conclusions:
- The Quantigen assay provides a reliable and accurate method for simultaneous T- and B-lymphocyte enumeration via light microscopy.
- This assay demonstrates utility in analyzing lymphocyte populations, including in cases of chronic lymphocytic leukemia.
- The Quantigen assay offers a viable alternative to existing enumeration techniques.