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Structure and function of intracellular proteoglycans.

D D Metcalfe, K F Austen

    Monographs in Allergy
    |January 1, 1979
    PubMed
    Summary
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    Intracellular proteoglycans act as a matrix within secretory granules, facilitating the storage and release of mediators. This suggests proteoglycans play a crucial role in cell signaling and extracellular regulation.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Immunology

    Background:

    • Proteoglycans are traditionally viewed for extracellular roles.
    • Emerging evidence highlights their presence within secretory granules.
    • Mast cells contain heparin, while other blood cells have less-characterized chondroitin sulfates.

    Purpose of the Study:

    • To investigate the role of intracellular proteoglycans in secretory granules.
    • To explore the function of chondroitin sulfates in peripheral blood cells.
    • To understand the storage and release mechanisms of chemical mediators.

    Main Methods:

    • Studied coupled activation-secretion in rat mast cells and human platelets.
    • Analyzed the release of proteoglycan-protein complexes.

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  • Examined the composition and bioavailability of granule constituents post-discharge.

    • Main Results:

    • Demonstrated the release of proteoglycan-protein complexes during cell activation.
    • Identified chondroitin sulfates as major proteoglycans in peripheral blood cells.
    • Observed differences in the bioavailability of rat mast cell granule constituents after discharge.

    Conclusions:

    • Intracellular proteoglycans serve as a granule matrix for mediator storage and transport.
    • Proteoglycans are essential for the regulated discharge of mediators.
    • Extracellular proteoglycans may possess regulatory functions in the microenvironment.