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  11. Pc4 Promotes Bladder Cancer Progression And Stemness By Directly Interacting With Sp1 To Transcriptionally Activate The Wnt5a/β-catenin Pathway.

PC4 promotes bladder cancer progression and stemness by directly interacting with Sp1 to transcriptionally activate the Wnt5a/β-catenin pathway.

Benhuang Yan1, Peng Luo2, Heping Qiu1

  • 1Department of Urology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing 401120, China.

Pathology, Research and Practice
|May 31, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Positive cofactor 4 (PC4) promotes bladder cancer progression by enhancing cancer stemness via the Wnt5a/β-catenin pathway. PC4 deficiency inhibits tumor growth and metastasis, offering a potential therapeutic target for bladder cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Bladder cancer presents a significant global health challenge with limited effective treatments.
  • Positive cofactor 4 (PC4) is implicated in various cancers, but its role in bladder cancer is not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of PC4 in bladder cancer.
  • To determine the association between PC4 expression and patient survival.

Main Methods:

  • Analysis of PC4 expression in bladder cancer tissues.
  • In vitro experiments assessing the impact of PC4 deficiency on cancer cell proliferation and metastasis.
  • RNA sequencing (RNA-seq) to identify molecular pathways involved.
  • Experimental validation of identified mechanisms, including protein-protein interactions and gene transcription.
Keywords:
Bladder cancerPositive cofactor 4Sp1Stemness

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Main Results:

  • PC4 is upregulated in bladder cancer and correlates with poorer patient survival.
  • PC4 deficiency significantly suppressed bladder cancer cell proliferation and metastasis.
  • PC4 reduction decreased the expression of cancer stemness markers (CD44, CD47, KLF4, c-Myc).
  • Activation of the Wnt5a/β-catenin pathway was identified as a key mechanism.
  • PC4 was found to directly interact with Sp1, promoting Wnt5a transcription.

Conclusions:

  • PC4 plays a crucial role in promoting bladder cancer progression and stemness.
  • The PC4/Sp1/Wnt5a axis is a critical pathway in bladder cancer pathogenesis.
  • Targeting PC4 presents a potential therapeutic strategy for bladder cancer treatment.
Wnt5a