Enhancing high-efficiency breeding and microbial microdroplet cultivation techniques for Ganoderma lucidum

Area of Science:

• Mycology
• Biotechnology
• Food Science

Background:

• Ganoderma lucidum is valued for bioactive compounds like polysaccharides and triterpenoids.
• Liquid fermentation of Ganoderma lucidum faces challenges in strain differentiation and stability.
• Developing improved strains is crucial for efficient industrial applications.

Purpose of the Study:

• To identify Ganoderma lucidum strains with enhanced biomass and triterpenoid production using novel mutation and screening techniques.
• To evaluate the stability and performance of the selected mutant strains in liquid fermentation.

Main Methods:

• Atmospheric room temperature plasma mutation was used for strain improvement.
• A microbial microdroplet culture system was employed for high-throughput screening.
• Shake flask cultivation and colorimetric detection were used to assess biomass and triterpenoid yield.
• Strain stability was assessed over five generations.

Main Results:

• Three mutant strains (YB05, YB09, YB18) showed accelerated growth and improved antagonism compared to the initial strain.
• YB05 and YB18 exhibited significantly greater biomass (26.33% and 17.85% increase, respectively).
• YB05 and YB18 demonstrated superior triterpenoid production (32.10% and 15.72% increase, respectively).
• The identified mutant strains maintained stability for at least five generations.

Conclusions:

• Atmospheric pressure, room temperature plasma mutation combined with microbial microdroplet culture is an effective system for Ganoderma lucidum strain improvement.
• This approach successfully identified stable, high-yielding Ganoderma lucidum strains for liquid fermentation.
• The developed methodology provides a promising pathway for obtaining advantageous strains for industrial applications.