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Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions
Published on: January 7, 2019
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Effect of Different Isolation Methods on Bioaerosol Distribution: An In Vitro Study
Mitchell E Westberg1, Richard L Gregory2, James E Jones3
1Pediatric Dentist in Private Practice, Albany, Ore., USA.
Pediatric Dentistry
|June 1, 2024
Summary
Dental dam isolation with high-volume suction during stainless steel crown preparations increased airborne bacteria. DryShield® evacuation systems effectively limited bioaerosol spread in operatories.
Area of Science:
- Dentistry
- Microbiology
- Infection Control
Background:
- Bioaerosols generated during dental procedures pose infection risks.
- Controlling airborne microorganisms is crucial for patient and dental team safety.
Purpose of the Study:
- To evaluate bioaerosol mitigation during stainless steel crown (SSC) preparations.
- To assess the distribution of Streptococcus mutans aerosols with different isolation methods.
Main Methods:
- In vitro study using melamine teeth with S. mutans biofilm.
- Three isolation methods tested: HVE with dental dam (DD), HVE with DryShield® (DS), and HVE alone.
- Aerosolized S. mutans collected on agar plates at five operatory locations.
Main Results:
- Dental dam with HVE significantly increased S. mutans aerosols at assistant, face shield, and patient locations compared to DryShield®.
- DryShield® significantly reduced aerosol spread compared to dental dam isolation.
- No significant differences in aerosol counts were found at the parent or rear delivery locations among methods.
Conclusions:
- Dental dam with HVE may increase bioaerosol proximity during SSC preparations.
- DryShield® evacuation systems show potential for effectively limiting bioaerosol spread in dental operatories.
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