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Related Experiment Video

Updated: Jun 24, 2025

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Protocol for electroporating and isolating murine (sub)ventricular zone cells for single-nuclei omics.

Jennie C Holmberg1, Victoria A Riley1, Aidan M Sokolov1

  • 1Department of Biological Sciences, Clemson University, Clemson, SC 29631, USA.

STAR Protocols
|June 1, 2024
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Summary

This study presents a protocol for in vivo genetic modification of mouse neural stem cells using electroporation. This method enables the study of neurological disorders by analyzing genetically modified cells and their progeny via single-nuclei omics.

Keywords:
Developmental biologyNeuroscienceStem Cells

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • In vivo genetic modification of neural stem cells is crucial for understanding neurological disorders.
  • Electroporation is a key technique for introducing genetic material into brain cells.

Purpose of the Study:

  • To provide a detailed protocol for electroporation of neonatal mouse ventricular neural stem cells.
  • To enable subsequent single-nuclei omics analysis of genetically modified cells and their progeny.

Main Methods:

  • Electroporation of neural stem cells in neonatal mouse ventricles.
  • Isolation and preparation of nuclei from modified cells and their progeny.
  • Single-nuclei omics techniques for downstream analysis.

Main Results:

  • Successful establishment of a protocol for in vivo electroporation of neural stem cells.
  • Demonstration of the feasibility of preparing nuclei for single-nuclei omics from electroporated cells.
  • Provides a foundation for studying the origins and pathogenesis of neurological disorders.

Conclusions:

  • The described protocol facilitates in vivo genetic modification of neural stem cells.
  • This method supports advanced omics analyses for neurological disorder research.
  • Offers a valuable tool for modeling the pathogenesis of neurological conditions.