Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Obesity01:24

Obesity

453
The Body Mass Index (BMI) is a numerical value derived from a person's weight and height, used to categorize individuals into weight ranges. It is calculated using the formula: weight in kilograms divided by height in meters squared. Obesity is a health condition characterized by excessive accumulation of adipose tissue that poses health risks, often diagnosed with a BMI ≥ 30. This excess fat storage occurs when surplus dietary calories are converted into triglycerides and stored in...
453

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Periodontal regenerative therapies with dental stem cells: from pulp-fiction to clinical application.

Frontiers of oral and maxillofacial medicine·2026
Same author

MT-MRI for detection of renal interstitial fibrosis in renovascular disease.

Scientific reports·2026
Same author

Renovascular disease induces functionally relevant, locus-specific alterations to DNA methylation and hydroxymethylation in swine scattered tubular-like cells.

American journal of physiology. Renal physiology·2026
Same author

miRNA family miR-29 inhibits PINK1-PRKN signaling via ATG9A.

Molecular neurodegeneration advances·2026
Same author

Mitochondrial-derived peptide MOTS-c activates metabolic signaling but blunts reparative function in human mesenchymal stromal cells.

Inflammation and regeneration·2026
Same author

Mapping Microvascular Flow via Radon Transform Ultrasound: Technical Advances and Pilot Application.

BME frontiers·2026

Related Experiment Video

Updated: Jun 24, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.1K

Obesity-driven mitochondrial dysfunction in human adipose tissue-derived mesenchymal stem/stromal cells involves

Alfonso Eirin1,2, Roman Thaler3, Logan M Glasstetter1

  • 1Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA.

Cell Death & Disease
|June 1, 2024
PubMed
Summary

Obesity impairs human mesenchymal stem cells (MSCs) by altering mitochondrial gene epigenetics, affecting their function and regenerative potential. Epigenetic modulation can restore MSC function in obese individuals.

More Related Videos

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

11.8K
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.1K

Related Experiment Videos

Last Updated: Jun 24, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

2.1K
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
10:28

Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue

Published on: July 17, 2018

11.8K
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.1K

Area of Science:

  • Epigenetics
  • Mitochondrial Biology
  • Stem Cell Biology

Background:

  • Obesity negatively impacts mesenchymal stem/stromal cell (MSC) function and tissue repair.
  • Mitochondria play a crucial role in MSC viability, proliferation, and differentiation.
  • Mechanisms underlying obesity-induced MSC dysfunction remain unclear.

Purpose of the Study:

  • To investigate if epigenetic alterations in mitochondria-related genes mediate obesity-driven dysfunction in human adipose-derived MSCs.
  • To explore the impact of obesity on the 5-hydroxymethylcytosine (5hmC) profile of mitochondrial genes in MSCs.
  • To assess the functional consequences of these epigenetic changes on MSCs.

Main Methods:

  • Harvested MSCs from obese and non-obese subjects.
  • Utilized hydroxymethylated DNA immunoprecipitation sequencing (hMeDIP-seq) and mRNA sequencing (mRNA-seq) to analyze 5hmC profiles and gene expression.
  • Evaluated MSC mitochondrial structure, function, metabolomics, proliferation, and neurogenic differentiation in vitro.
  • Assessed effects of epigenetic modulation on MSCs.

Main Results:

  • Obesity altered 5hmC profiles in nuclear-encoded mitochondrial genes (99 hyper, 150 hypo).
  • Integrated analysis revealed overlapping changes in 5hmC and mRNA for genes involved in ATP production, redox, proliferation, and metabolism.
  • Obese-MSCs showed impaired mitochondrial function, reduced fatty acid metabolism, and decreased neurogenic differentiation, which were improved by epigenetic modulation.

Conclusions:

  • Obesity induces epigenetic modifications in mitochondria-related genes within human adipose-derived MSCs.
  • These epigenetic changes correlate with mitochondrial structural/functional deficits and impaired MSC regenerative capacity.
  • Findings suggest potential therapeutic targets for enhancing MSC function in obesity.