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Pump-induced insulin aggregation. A problem with the Biostator.

J R Brennan, S S Gebhart, W G Blackard

    Diabetes
    |April 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

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    Plasma insulin loss in Biostator studies occurs at slow infusion rates due to heat-induced aggregation, impacting clamp study accuracy. Researchers must check systems for this flow-rate-dependent insulin alteration.

    Area of Science:

    • Endocrinology
    • Biomedical Engineering

    Background:

    • Prolonged insulin euglycemic clamp studies utilize the Biostator for precise insulin delivery.
    • Observed falls in plasma immunoreactive insulin (IRI) despite stable C-peptide levels raised concerns about the Biostator's insulin delivery system.

    Purpose of the Study:

    • To meticulously evaluate the Biostator's insulin delivery system for observed insulin loss during clamp studies.

    Main Methods:

    • Investigated insulin loss using labeled insulin and various analytical techniques including gel filtration, electrophoresis, and centrifugation.
    • Assessed the impact of infusion flow rate and time on insulin activity within the Biostator system.

    Main Results:

    • Significant loss of immunoreactive and biologically active insulin occurred at slow infusion rates (e.g., <4.2 ml/h) after 6 hours.

    Related Experiment Videos

  • Insulin loss was attributed to precipitation out of solution, confirmed by centrifugation, not adsorption to tubing.
  • Insulin alteration was localized to the pump assembly, likely due to heat-induced aggregation, and was flow rate-dependent.

    • Conclusions:

    • The Biostator's insulin delivery system can alter insulin activity, particularly at low flow rates and over time.
    • This phenomenon, likely heat-induced aggregation, can profoundly influence data accuracy in low-dose insulin clamp studies.
    • Investigators using the Biostator must carefully examine their systems for this time- and flow rate-dependent alteration of insulin.