Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

Colloidal precipitates

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Subject injury and police use of force in mental health crises.

International journal of law and psychiatry·2025

Same author

Dairy Foods: A Matrix for Human Health and Precision Nutrition-Introduction.

Journal of dairy science·2025

Same author

An integrated biological effects assessment of the discharge water into the Sunndalsfjord from an aluminium smelter.

The Science of the total environment·2023

Same author

Developmental stage and morphology of the competent blastocyst are associated with sex of the child but not with other obstetric outcomes: a multicenter cohort study.

Human reproduction (Oxford, England)·2022

Same author

Necessary duration of follow-up to assess complications of mesh in hernia surgery: a time-lapse study based on 460 explants.

Hernia : the journal of hernias and abdominal wall surgery·2020

Same author

Procedure-specific assessment tool for flexible pharyngo-laryngoscopy: gathering validity evidence and setting pass-fail standards.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2018

Search research articles

Related Experiment Video

Updated: Jun 24, 2025

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

Enhancing casein micelle dissociation in diluted skim milk systems using combined processing techniques.

D R Stroinski¹, K Petersen¹, G E Lewis¹

¹Department of Animal and Food Science, University of Wisconsin-River Falls, River Falls, WI 54022.

Journal of Dairy Science

|June 2, 2024

Summary

This study explored casein micelle dissociation in skim milk using ethanol or emulsifying salts combined with ultra-high-pressure homogenization (UHPH). Optimized conditions achieved significant micelle breakdown, creating potential for novel food ingredient applications.

Keywords:

casein emulsifying salts ethanol high-pressure homogenization

More Related Videos

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids

Published on: November 18, 2022

Extraction of Plant-based Capsules for Microencapsulation Applications

Extraction of Plant-based Capsules for Microencapsulation Applications

Published on: November 9, 2016

Related Experiment Videos

Last Updated: Jun 24, 2025

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality

Published on: April 7, 2023

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids

Published on: November 18, 2022

Extraction of Plant-based Capsules for Microencapsulation Applications

Extraction of Plant-based Capsules for Microencapsulation Applications

Published on: November 9, 2016

Area of Science:

Food Science
Dairy Science
Physical Chemistry

Background:

Casein micelles are complex colloidal structures in milk.
Understanding casein micelle dissociation is key for modifying milk functionalities.
Existing methods for dissociation have limitations.

Purpose of the Study:

To evaluate casein micelle dissociation in diluted skim milk.
To investigate the combined effects of solvent/emulsifying salt treatments and ultra-high-pressure homogenization (UHPH).
To explore potential applications of dissociated casein systems.

Main Methods:

Diluted skim milk solutions were treated with varying ethanol concentrations (0-60%) or emulsifying salts (sodium hexametaphosphate, sodium citrate).
Treatments were combined with ultra-high-pressure homogenization (UHPH) at pressures ranging from 100-300 MPa.
Casein micelle dissociation was assessed via changes in sample absorbance, particle size, and hydrophobicity.

Main Results:

High ethanol concentrations (40-60%) at elevated temperatures (45-65°C) combined with UHPH (≥200 MPa) significantly dissociated casein micelles.
Emulsifying salts, specifically sodium hexametaphosphate (≥1 mM) and sodium citrate (≥10 mM), also induced dissociation, enhanced by UHPH (≥200 MPa).
Dissociation was evidenced by reduced absorbance, altered particle size distribution, and increased hydrophobicity.

Conclusions:

Both solvent-based (ethanol) and emulsifying salt-based methods effectively dissociate casein micelles when coupled with UHPH.
Optimized processing parameters enhance the extent of casein micelle dissociation.
These findings suggest potential for using dissociated casein systems as functional ingredients or encapsulation agents in the food industry.