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Bioinformatics Analysis Reveals HIST1H2BH as a Novel Diagnostic Biomarker for Atrial Fibrillation-Related Cardiogenic
Wenbing Jiang1, Lelin Jiang2, Xiaoli Zhao3
1Department of Cardiology, Wenzhou Integrated Traditional Chinese and Western Medicine Hospital, No.75 Jinxiu Road, Lucheng District, Wenzhou, 325000, Zhejiang Province, People's Republic of China. jiangwb919@wmu.edu.cn.
Atrial fibrillation (AF) can lead to stroke. Researchers identified HIST1H2BH as a key biomarker for diagnosing AF-related cerebral embolism (AF-CE), offering new diagnostic potential.
Area of Science:
- Biomarker Discovery
- Genomics
- Cardiovascular Research
Background:
- Atrial fibrillation (AF) is a major risk factor for cerebral embolism.
- Current diagnostic methods for AF-related cerebral embolism (AF-CE) require improvement.
- Identifying novel biomarkers is crucial for early detection and management of AF-CE.
Purpose of the Study:
- To discover novel diagnostic biomarkers for atrial fibrillation-related cerebral embolism (AF-CE).
- To analyze gene expression data from AF and stroke patient cohorts.
- To validate potential biomarkers using meta-analysis and statistical modeling.
Main Methods:
- Examination of multiple Gene Expression Omnibus (GEO) datasets.
- Meta-analysis of RNA sequencing data to identify differentially expressed genes.
- LASSO regression and Receiver Operating Characteristic (ROC) curve analysis for biomarker selection and validation.
- Gene Ontology (GO) and KEGG pathway enrichment analysis.
- Protein-protein interaction (PPI) network analysis.
Main Results:
- Differential gene expression analysis identified 16 upregulated and 4 downregulated genes in stroke patient blood samples.
- Meta-analysis confirmed varied expression in eight genes; LASSO regression pinpointed five key genes.
- HIST1H2BH was identified as a characteristic gene for AF-CE, with high diagnostic accuracy confirmed by ROC analysis.
- Active pathways included integrin signaling, platelet activation, ECM interactions, and PI3K-Akt.
- HIST1H2BH showed interaction with ITGA2B and links with NK cells and eosinophils.
Conclusions:
- HIST1H2BH is a promising diagnostic biomarker for atrial fibrillation-related cerebral embolism (AF-CE).
- This biomarker may influence platelet activation and thrombus formation, contributing to embolism.
- Further research into HIST1H2BH's role can enhance AF-CE diagnosis and therapeutic strategies.
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