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Related Experiment Video

Updated: Jun 24, 2025

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Minimizing IP issues associated with gene constructs encoding the Bt toxin - a case study.

Md Mahmudul Hassan^1,2, Francis Tenazas¹, Adam Williams¹

¹School of Biosciences, University of Melbourne, Parkville, VIC, 3010, Australia.

BMC Biotechnology

|June 2, 2024

Summary

This summary is machine-generated.

Modified Bacillus thuringiensis (Bt) Cry1B and Cry1C genes show high expression in Brassica plants, leading to 100% mortality in diamondback moth larvae. These insect-resistant GM crops can be developed with reduced licensing costs.

Keywords:

Bacillus thuringiensis Cry1 M Cry1B Cry1C Bt toxin Diamondback moth Gene stacking Insecticidal gene

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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells

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Published on: August 20, 2018

Area of Science:

Agricultural Science
Molecular Biology
Genetics

Background:

Development of genetically engineered Brassicas (cabbage, cauliflower, canola) expressing Bacillus thuringiensis (Bt) insecticidal toxins.
Modified Cry1B and Cry1C genes (Cry1B^M, Cry1C^M) designed to minimize licensing costs and optimize plant activity.
Publicly funded initiative targeting Indian and Australian farmers.

Purpose of the Study:

Assess insecticidal efficacy of modified Cry1B^M and Cry1C^M genes in Brassica.
Evaluate expression levels driven by single (S4/S7) or double (S4S4/S7S7) subterranean clover stunt virus (SCSV) promoters.
Determine suitability for developing cost-effective, insect-resistant genetically modified (GM) crops.

Main Methods:

Constructs with modified Cry1B^M/Cry1C^M genes introduced into Arabidopsis thaliana.
Expression driven by single or double SCSV promoters.
Insecticidal bioassays using Diamondback moth (Plutella xylostella) larvae.

Main Results:

High-level Cry1B^M/Cry1C^M expression observed in transgenic Arabidopsis, with Cry1C^M protein accumulation ranging from 5.18 to 176.88 µg/g dry weight.
No correlation found between single/double SCSV promoters and gene expression levels.
100% mortality of first instar Diamondback moth larvae fed on transgenic leaves; minimal leaf damage (0.125 vs. 4.2).

Conclusions:

Modified Cry1 genes are suitable for developing insect-resistant GM crops.
Construct components appear usable without further licensing (except PAT in USA), significantly reducing development costs.
Potential for cost-effective insect resistance in future GM crop plants.