Rapidly inducible yeast surface display for antibody evolution with OrthoRep

Area of Science:

• Biotechnology
• Molecular Biology
• Immunology

Background:

• Yeast surface display is a powerful tool for antibody evolution.
• Current methods like AHEAD (autonomous hypermutation yeast surface display) can be slow due to long induction times.
• Optimizing induction systems is crucial for accelerating antibody discovery.

Conclusions:

• The synthetic β-estradiol induction system offers a faster and efficient alternative for yeast surface display.
• This optimization accelerates the AHEAD platform's capability for rapid antibody evolution and discovery.
• The improved system holds promise for developing potent and specific therapeutic antibodies more efficiently.