Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GRP78 expression and prognostic significance in patients with pancreatic ductal adenocarcinoma treated with neoadjuvant therapy versus surgery first.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]·2021
Same author

Elucidation of Tumor-Stromal Heterogeneity and the Ligand-Receptor Interactome by Single-Cell Transcriptomics in Real-world Pancreatic Cancer Biopsies.

Clinical cancer research : an official journal of the American Association for Cancer Research·2021
Same author

Early detection of pancreatic cancer: current state and future opportunities.

Current opinion in gastroenterology·2021
Same author

PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinoma.

Nature communications·2021
Same author

Longitudinal assessment of lung clearance index to monitor disease progression in children and adults with cystic fibrosis.

Thorax·2021
Same author

EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart.

Molecular cell·2021

Related Experiment Video

Updated: Jun 24, 2025

Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients
07:43

Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients

Published on: September 25, 2018

10.6K

A Rigorous Multi-Laboratory Study of Known PDAC Biomarkers Identifies Increased Sensitivity and Specificity Over

Brian Haab, Lu Qian, Ben Staal

    Biorxiv : the Preprint Server for Biology
    |June 3, 2024
    PubMed
    Summary

    A new blood test combining CA19-9 with other biomarkers significantly improves early detection of pancreatic cancer (PDAC). This approach enhances sensitivity and specificity for pancreatic ductal adenocarcinoma surveillance.

    More Related Videos

    Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
    06:53

    Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

    Published on: June 8, 2019

    8.6K
    Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
    05:44

    Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

    Published on: November 5, 2020

    4.3K

    Related Experiment Videos

    Last Updated: Jun 24, 2025

    Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients
    07:43

    Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients

    Published on: September 25, 2018

    10.6K
    Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
    06:53

    Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

    Published on: June 8, 2019

    8.6K
    Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma
    05:44

    Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

    Published on: November 5, 2020

    4.3K

    Area of Science:

    • Oncology
    • Biomarker Discovery
    • Clinical Diagnostics

    Background:

    • Early detection of pancreatic ductal adenocarcinoma (PDAC) via blood tests is critical but challenging.
    • Existing biomarkers like CA19-9 have limitations in sensitivity and specificity for early-stage PDAC.
    • Performance of previously identified PDAC biomarkers in combination remains largely unknown.

    Approach:

    • A multi-laboratory, coordinated case/control study utilized blinded training (132 samples) and validation (295 samples) sets.
    • Candidate biomarker panels were identified using training data, focusing on combinations of CA19-9 with novel glycan and protein biomarkers.
    • Fixed panels were rigorously tested on the independent validation set to assess performance.

    Key Points:

    • A panel including CA19-9 and the glycan biomarker CA199.STRA significantly improved sensitivity from 0.44 to 0.71 (at 0.98 specificity).
    • Combining CA19-9 with protein biomarker LRG1 and CA199.STRA enhanced sensitivity to 0.89 (at 0.65 specificity).
    • Validated biomarker panels, including those without CA19-9, demonstrated significantly improved early PDAC detection capabilities.

    Conclusions:

    • Coordinated studies of previously discovered biomarkers are effective for validating diagnostic panels.
    • Identified biomarker combinations substantially increase the sensitivity and specificity of early-stage PDAC detection.
    • This research provides a robust foundation for developing improved blood-based surveillance for pancreatic cancer.