Beauveria Bassiana Amylase-Polygalacturonase Production Using Lignocellulosic Biomass and Application in Juice...

Area of Science:

• Biotechnology
• Enzyme Technology
• Industrial Microbiology

Background:

• The enzyme industry seeks non-pathogenic biocatalysts utilizing inexpensive nutrient sources.
• Beauveria bassiana, an endophytic fungal entomopathogen, is non-pathogenic and can secrete bioproducts using lignocellulosic biomass.

Purpose of the Study:

• To optimize the production of amylase and polygalacturonase by B. bassiana using agricultural residues.
• To evaluate the industrial potential of these enzymes in fruit juice clarification.

Main Methods:

• Screening of seven agro-residues for enzyme production by B. bassiana SAN01.
• Statistical optimization using Plackett Burman Design (PBD) and Central Composite Design (CCD).
• Partial purification of enzymes and application in pineapple juice clarification.

Main Results:

• Wheat bran was identified as the optimal substrate for enzyme production.
• Optimized conditions (30 °C, pH 6.0, ~40 g.L-1 wheat bran) significantly increased enzyme yields (179% for amylase, 187% for polygalacturonase).
• Optimal clarification of pineapple juice (~90%) achieved at 35 °C after 120 min incubation.

Conclusions:

• B. bassiana can produce high yields of amylase and polygalacturonase from agricultural residues, a novel finding for this entomopathogen.
• Achieved enzyme production levels are the highest reported for these enzymes from any endophytic fungal entomopathogen.
• Demonstrates B. bassiana's potential for industrial applications beyond its biopesticide role.