Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Sanger Sequencing01:57

Sanger Sequencing

754.0K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.0K
Next-generation Sequencing03:00

Next-generation Sequencing

88.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.6K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Signal Sequences and Sorting Receptors01:41

Signal Sequences and Sorting Receptors

5.3K
Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid <i>de novo</i> assembly of animal-microbe biofilter to mitigate seabed methane leakage.

National science review·2026
Same author

Isolation of genome-predicted Caldatribacterium (Atribacterota) reveals pervasive microbial cultivation problem due to folate precipitation.

Nature communications·2026
Same author

An ANIr-based methodology to determine if two sequence-discrete populations are identical and identify cosmopolitan prokaryotic populations.

ISME communications·2026
Same author

Hunting for new lineages and mechanisms of life.

Nature microbiology·2026
Same author

Exploring the lipoproteome of Parageobacillus thermoglucosidasius.

Access microbiology·2026
Same author

<i>Apolloniradiicaulis salifontis</i> gen. nov., sp. nov., a New Prosthecate Aerobic Anoxygenic Phototroph Isolated from Lake Winnipegosis Region Salt Springs.

Microorganisms·2026
Same journal

fCUT&Tag-Seq: An optimized CUT&Tag-based method for high-resolution profiling of histone modifications and chromatin-binding proteins in fungi.

mLife·2026
Same journal

An infection and pathogenesis mouse model of SARS-CoV-2-related pangolin coronavirus GX_P2V(short_3UTR).

mLife·2026
Same journal

The synthetic estradiol analog E0703 enhances <i>Akkermansia muciniphila</i> growth for radiation-induced intestinal damage repair.

mLife·2026
Same journal

Osmotic signaling governs sunscreen biosynthesis to safeguard desert cyanobacteria against desiccation.

mLife·2026
Same journal

Quinolone tolerance in <i>Escherichia coli</i> due to defects in the adenosine ribonucleotides <i>de novo</i> biosynthesis pathway.

mLife·2026
Same journal

Emerging role of metagenomic next-generation sequencing in infectious disease diagnostics: Clinical integration and future directions.

mLife·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K

Why and how to use the SeqCode.

William B Whitman1, Maria Chuvochina2, Brian P Hedlund3

  • 1Department of Microbiology University of Georgia Athens Georgia USA.

Mlife
|June 3, 2024
PubMed
Summary
This summary is machine-generated.

The new SeqCode allows naming prokaryotes using genome sequences, including uncultured species. This system ensures a single, unified name for each prokaryotic species, whether cultured or not.

Keywords:
SeqCodegenome sequencesmetagenome‐assembled genomesnomenclature

More Related Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.1K

Related Experiment Videos

Last Updated: Jun 24, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

2.0K
Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.1K

Area of Science:

  • Microbiology
  • Bioinformatics
  • Taxonomy

Background:

  • The International Code of Nomenclature of Prokaryotes (ICNP) traditionally requires type strain deposition in culture collections.
  • A vast diversity of prokaryotes remains uncultured, limiting their formal taxonomic description.
  • Genome sequencing is increasingly used for prokaryotic identification and classification.

Purpose of the Study:

  • Introduce the SeqCode (Code of Nomenclature of Prokaryotes Described from Sequence Data) as a novel system for prokaryotic nomenclature.
  • Enable the naming of prokaryotes, particularly uncultured species, using genome sequences as type material.
  • Establish a unified taxonomic framework for both cultured and uncultured prokaryotes.

Main Methods:

  • Genome sequences serve as nomenclatural types for prokaryotic species under the SeqCode.
  • The SeqCode operates with rules similar to the ICNP but omits the requirement for type strain deposition.
  • An online Registry (seqco.de) facilitates the valid publication of names starting January 1, 2022.

Main Results:

  • The SeqCode allows permanent naming of prokaryotes from environmental DNA and those difficult to culture.
  • Validly published names under the SeqCode compete with ICNP names for priority post-2022.
  • A single, unique name is established for each prokaryotic species, integrating cultured and uncultured organisms.

Conclusions:

  • The SeqCode is essential for naming the majority of prokaryotic species due to the prevalence of uncultured organisms.
  • It facilitates effective communication and the development of unified prokaryotic taxonomies.
  • The SeqCode Community encourages participation in the ongoing development of this nomenclature system.