Related Experiment Video
Updated: Jun 24, 2025
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
Published on: February 19, 2019
Dynamic Changes in Soluble Triggering Receptor Expressed on Myeloid Cells-1 in Sepsis with Respect to Antibiotic
Young Woo Um1, Inwon Park1, Jae Hyuk Lee1
1Department of Emergency Medicine, Seoul National University Bundang Hospital, Seongnam-si, Gyeonggi-do, Korea.
Purpose:
Proper antibiotic administration is crucial for sepsis management. Given the escalating incidence of antimicrobial resistance, there is a pressing need for indicators of antimicrobial susceptibility with short turnaround times. This study aimed to investigate the potential of soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) as an early biomarker for in vivo antibiotic susceptibility in patients with sepsis.
Patients And Methods:
We conducted a retrospective analysis of plasma samples from patients enrolled in a pre-established study designed to investigate prognostic biomarkers in patients with sepsis or septic shock. Baseline and 6 h sTREM-1 levels were examined using enzyme-linked immunosorbent assays. The primary outcome of the study was the comparison of percentage changes in sTREM-1 levels at the 6 h relative to baseline with respect to antibiotic susceptibility.
Results:
Of the 596 patients enrolled in the pre-established study, 29 with a median age of 75.8 and a 28-day mortality rate of 17.2% were included in the present analysis. Among these patients, 24 were classified into the susceptible group, whereas the remaining five were classified into the resistant group. The trend in plasma sTREM-1 levels differed with respect to antibiotic susceptibility. Moreover, percentage change in sTREM-1 levels at the 6 h relative to baseline was significantly higher in the resistant group (P = 0.028).
Conclusion:
The trend in plasma sTREM-1 levels in patients with sepsis differed with respect to antibiotic susceptibility, with a higher percentage change in patients treated with inappropriate antibiotics. These findings indicate the potential utility of sTREM-1 as an early biomarker of antibiotic susceptibility.
Insights
Soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) levels changed differently in sepsis patients based on antibiotic susceptibility. A higher sTREM-1 increase indicated resistance, suggesting sTREM-1 as an early biomarker for antibiotic response.
Area of Science:
- Biochemistry
- Immunology
- Clinical Medicine
Background:
- Sepsis management requires timely and appropriate antibiotic administration.
- Antimicrobial resistance necessitates rapid indicators of antibiotic susceptibility.
- Current diagnostic methods for antibiotic susceptibility have lengthy turnaround times.
Purpose of the Study:
- To evaluate soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) as an early predictive biomarker for in vivo antibiotic susceptibility in sepsis patients.
- To assess the utility of sTREM-1 levels in distinguishing between susceptible and resistant bacterial infections in sepsis.
Main Methods:
- Retrospective analysis of plasma samples from sepsis patients.
- Measurement of sTREM-1 levels at baseline and 6 hours post-antibiotic administration using enzyme-linked immunosorbent assays.
- Comparison of percentage changes in sTREM-1 levels in relation to antibiotic susceptibility.
Main Results:
- Twenty-nine patients were included in the analysis (24 susceptible, 5 resistant).
- Plasma sTREM-1 levels showed differing trends based on antibiotic susceptibility.
- A significantly higher percentage increase in sTREM-1 at 6 hours was observed in the resistant group (P=0.028).
Conclusions:
- The trend of plasma sTREM-1 levels differs between susceptible and resistant infections in sepsis.
- A greater percentage change in sTREM-1 levels at 6 hours suggests poorer antibiotic susceptibility.
- sTREM-1 shows potential as an early biomarker for assessing antibiotic susceptibility in sepsis management.
