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Related Experiment Video

Updated: Dec 19, 2025

Establishing a Severe Corneal Inflammation Model in Rats Based on Corneal Epithelium Curettage Combined with Corneal Sutures

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ROS-responsive celastrol-nanomedicine alleviates inflammation for dry eye disease.

Bingbing Cui^1,2, Nan Zhang¹, Wei Zhang^1,2

¹Henan Eye Hospital, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou 450003, People's Republic of China.

|June 3, 2024

Summary

This study developed a novel nanomedicine for dry eye disease (DED) treatment. The reactive oxygen species (ROS) sensitive micelles improve celastrol delivery, effectively reducing ocular inflammation and promoting eye repair.

Keywords:

ROS sensitive celastrol dry eye disease inflammation nanomedicine

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Published on: August 4, 2022

Area of Science:

Ophthalmology
Nanomedicine
Pharmacology

Background:

Dry eye disease (DED) is a prevalent global condition causing significant eye discomfort and potential vision loss.
Increasing DED incidence is linked to high electronic device usage.
Celastrol (Cel) shows therapeutic promise for ocular disorders but suffers from poor solubility and short half-life, hindering its clinical application.

Purpose of the Study:

To develop a celastrol (Cel)-loaded, reactive oxygen species (ROS)-sensitive polymeric micelle for enhanced dry eye disease (DED) treatment.
To investigate the solubility, stability, and therapeutic efficacy of the novel nanomedicine in preclinical models of DED.

Main Methods:

Fabrication of ROS-sensitive polymeric micelles for encapsulating celastrol (Cel).
In vitro evaluation of Cel-loaded micelles, including solubility, ROS-responsive release, and anti-inflammatory effects.
In vitro and in vivo assessment of the nanomedicine's efficacy in alleviating DED symptoms, including inflammation, corneal repair, and goblet cell restoration.

Main Results:

The developed polymeric micelles significantly improved Cel's water solubility and demonstrated ROS-responsive release characteristics.
Cel-loaded nanomedicine effectively inhibited TLR4 signaling activation and reduced pro-inflammatory cytokine expression in DED models.
In vivo studies confirmed the nanomedicine's ability to reduce ocular inflammation, promote corneal epithelial repair, and restore tear secretion.

Conclusions:

Cel-loaded ROS-sensitive polymeric micelles offer a promising new therapeutic strategy for dry eye disease (DED).
The nanomedicine effectively delivers celastrol, alleviates ocular inflammation, and supports ocular surface restoration.
This approach provides a viable new treatment option for patients suffering from dry eye disease (DED).