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Measles virus P gene codes for two proteins
Abstract:
The entirety of the phosphoprotein gene of measles virus has been sequenced. The gene is composed of 1,657 nucleotides and specifies a 507-amino-acid protein (P). A second overlapping reading frame was predicted from the sequence and specifies a 186-amino-acid protein (C). Through the use of antisynthetic peptide antibodies, we show that both proteins are expressed in virally infected cells. Both proteins are expressed from a functionally bicistronic mRNA through independent initiation of ribosomes at the respective AUG codons. Using immunofluorescent microscopy, we localized the C protein in the nucleus and in cytoplasmic inclusions within the infected cells.
Insights
Measles virus phosphoprotein gene sequencing revealed two proteins, P and C, expressed from a single mRNA. Both measles virus proteins are confirmed to be present in infected cells.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- The measles virus phosphoprotein (P) gene is crucial for viral replication.
- Understanding the full coding potential of viral genes is essential for developing antiviral strategies.
Purpose of the Study:
- To sequence the entire phosphoprotein gene of the measles virus.
- To identify and characterize all proteins encoded by this gene.
- To investigate the expression and localization of these proteins in infected cells.
Main Methods:
- DNA sequencing of the measles virus phosphoprotein gene.
- Bioinformatic analysis to predict protein sequences and reading frames.
- Antibody-based detection of expressed proteins in infected cells.
- Immunofluorescent microscopy for protein localization.
Main Results:
- The measles virus phosphoprotein gene consists of 1,657 nucleotides.
- Two proteins, P (507 amino acids) and C (186 amino acids), are encoded by the gene.
- Both P and C proteins are expressed in measles virus-infected cells.
- Expression occurs from a bicistronic mRNA via independent initiation at AUG codons.
- The C protein is localized to the nucleus and cytoplasmic inclusions.
Conclusions:
- The measles virus phosphoprotein gene encodes two distinct proteins, P and C.
- These proteins are co-expressed from a single mRNA molecule.
- The localization of the C protein suggests its involvement in nuclear and cytoplasmic viral processes.