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Measles virus P gene codes for two proteins.

W J Bellini, G Englund, S Rozenblatt

    Journal of Virology
    |March 1, 1985
    PubMed
    Summary

    Measles virus phosphoprotein gene sequencing revealed two proteins, P and C, expressed from a single mRNA. Both measles virus proteins are confirmed to be present in infected cells.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Genetics

    Background:

    • The measles virus phosphoprotein (P) gene is crucial for viral replication.
    • Understanding the full coding potential of viral genes is essential for developing antiviral strategies.

    Purpose of the Study:

    • To sequence the entire phosphoprotein gene of the measles virus.
    • To identify and characterize all proteins encoded by this gene.
    • To investigate the expression and localization of these proteins in infected cells.

    Main Methods:

    • DNA sequencing of the measles virus phosphoprotein gene.
    • Bioinformatic analysis to predict protein sequences and reading frames.
    • Antibody-based detection of expressed proteins in infected cells.
    • Immunofluorescent microscopy for protein localization.

    Main Results:

    • The measles virus phosphoprotein gene consists of 1,657 nucleotides.
    • Two proteins, P (507 amino acids) and C (186 amino acids), are encoded by the gene.
    • Both P and C proteins are expressed in measles virus-infected cells.
    • Expression occurs from a bicistronic mRNA via independent initiation at AUG codons.
    • The C protein is localized to the nucleus and cytoplasmic inclusions.

    Conclusions:

    • The measles virus phosphoprotein gene encodes two distinct proteins, P and C.
    • These proteins are co-expressed from a single mRNA molecule.
    • The localization of the C protein suggests its involvement in nuclear and cytoplasmic viral processes.

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