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Related Experiment Video

Updated: Jun 24, 2025

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

Published on: December 23, 2022

Bioprinted Multi-Composition Array Mimicking Tumor Microenvironments to Evaluate Drug Efficacy with Multivariable

Gihyun Lee¹, Soo Jee Kim¹, Je-Kyun Park^1,2,3

¹Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Advanced Healthcare Materials

|June 3, 2024

Summary

This study introduces a novel bioprinted tumor microenvironment (TME) array on a microfluidic chip. This platform enables simultaneous drug efficacy testing across multiple conditions, improving TME research.

Keywords:

bioprinting drug screening microfluidic substrate multivariable analysis tumor microenvironment

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Last Updated: Jun 24, 2025

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

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Published on: December 23, 2022

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Published on: July 15, 2015

Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices

Measuring Real-time Drug Response in Organotypic Tumor Tissue Slices

Published on: May 2, 2020

Area of Science:

Biomedical Engineering
Cancer Research
Microfluidics

Background:

Current organ-on-a-chip models struggle to replicate complex in vivo tumor microenvironments (TMEs).
There is a need for advanced platforms to test drug efficacy under diverse experimental conditions simultaneously.

Purpose of the Study:

To develop a novel microfluidic device for constructing a multi-composition tumor array.
To mimic in vivo transport phenomena within TMEs and enable simultaneous drug efficacy evaluation.

Main Methods:

Bioprinting of 36 individual TME models with three different compositions on a microfluidic substrate.
Testing TME models under four varying drug concentrations.
Utilizing microfluidics for precise compartmentalization and observing self-organized vascular endothelial barriers.

Main Results:

The developed TME array successfully mimics complex transport phenomena.
Precise compartmentalization led to self-organized vascular endothelial barriers around cancer spheroids.
The platform allowed for multivariable screening of model complexity, replicates, and drug concentrations.

Conclusions:

Integrating bioprinting with microfluidics offers a powerful approach for comprehensive drug response evaluation in diverse TME conditions.
This novel platform overcomes limitations of current organ-on-a-chip technologies for TME research.