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Updated: Jun 24, 2025

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

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P53: A key player in diverse cellular processes including nuclear stress and ribosome biogenesis, highlighting

Gazmend Temaj1, Silvia Chichiarelli2, Pelin Telkoparan-Akillilar3

  • 1Faculty of Pharmacy, College UBT, 10000 Prishtina, Kosovo.

Biochemical Pharmacology
|June 3, 2024
PubMed

Insights

The tumor suppressor protein p53 regulates cellular processes and responds to stress. Mutations in p53 are linked to cancer and metabolic disorders like diabetes.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Oncology

Background:

  • Tumor suppressor proteins, like p53, are crucial transcription factors regulating vital cellular processes including apoptosis, DNA repair, cell cycle, senescence, and metabolism.
  • p53 acts as a central node in cellular stress response, mediating gene expression changes in response to diverse stimuli such as hypoxia, DNA damage, nutrient deprivation, and oncogene activation.

Purpose of the Study:

  • This review aims to summarize current knowledge on the molecular mechanisms and functions of p53.
  • It will explore p53's role in various cellular pathways and processes.
  • The review will also discuss the implications of p53 dysfunction in human health and diseases, including cancer and metabolic disorders.

Main Methods:

  • This review synthesizes existing scientific literature and data.
  • It focuses on molecular mechanisms and functional pathways involving p53.
  • The analysis covers p53's interactions, post-translational modifications, and non-coding RNA regulation.

Main Results:

  • Mutations or inactivation of p53 are frequently observed in many cancers, leading to loss of tumor suppression and gain of oncogenic functions.
  • p53 plays a significant role in the pathogenesis of metabolic disorders, including diabetes, obesity, and fatty liver disease.
  • p53 modulates protein activity and signaling pathways through protein-protein interactions, post-translational modifications, and non-coding RNAs.

Conclusions:

  • p53 is a critical regulator of cellular processes and stress responses with profound implications for human health.
  • Dysregulation of p53 contributes to cancer development and metabolic diseases.
  • Further understanding of p53's molecular functions is essential for developing therapeutic strategies.
Keywords:
CancerDrugsNuclear stressRibosome biogenesisp53p53 inhibitorsp53 mutation

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