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Antibiotic Selection

Antibiotic Selection

Bacterial Signaling

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...

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Related Experiment Video

Updated: Jun 24, 2025

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

β-lactamase expression induces collateral sensitivity in Escherichia coli.

Cristina Herencias^1,2, Laura Álvaro-Llorente³, Paula Ramiro-Martínez³

¹Servicio de Microbiología, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Hospital Universitario Ramón y Cajal, Madrid, Spain. cherodr@gmail.com.

Nature Communications

|June 3, 2024

Summary

Antimicrobial resistance (AMR) is a growing threat. This study reveals that resistance to beta-lactams can unexpectedly increase susceptibility to colistin and azithromycin, offering new strategies against mobile AMR.

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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

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Published on: February 1, 2018

Related Experiment Videos

Last Updated: Jun 24, 2025

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

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Published on: May 23, 2021

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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Antimicrobial resistance (AMR) genes are spreading rapidly, diminishing antibiotic effectiveness, particularly in Enterobacterales.
Beta-lactamases are a common AMR mechanism in Enterobacterales, often linked to mobile genetic elements, necessitating novel countermeasures.
Collateral sensitivity (CS) offers a potential strategy to combat AMR by exploiting increased susceptibility to one drug upon resistance to another, but typically arises from chromosomal mutations.

Purpose of the Study:

To investigate collateral sensitivity (CS) responses induced by the acquisition of mobile antibiotic resistance plasmids.
To determine if the expression of specific beta-lactamase genes can be therapeutically exploited to counteract mobile AMR.
To assess the prevalence of beta-lactamase-induced CS across different Enterobacterales strains.

Main Methods:

Dissected the CS response elicited by a prevalent antibiotic resistance plasmid carrying the blaOXA-48 gene.
Tested the susceptibility of multiple, phylogenetically distinct E. coli strains expressing various clinically relevant mobile beta-lactamases.
Combined experimental data with large-scale surveillance data from thousands of antibiotic susceptibility tests.

Main Results:

Expression of the beta-lactamase gene blaOXA-48 induced collateral sensitivity to colistin and azithromycin.
Other mobile beta-lactamases also elicited similar CS responses in diverse E. coli strains.
Beta-lactamase-induced CS was found to be pervasive across Enterobacterales.

Conclusions:

The physiological consequences of beta-lactamase expression can be harnessed for therapeutic benefit.
This discovery opens avenues for designing targeted therapies to combat or mitigate the impact of mobile AMR.
Leveraging beta-lactamase-induced collateral sensitivity offers a promising strategy against the global threat of antimicrobial resistance.