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Histological-Based Stainings Using Free-Floating Tissue Sections
Published on: August 25, 2020
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TSWIFT, a novel method for iterative staining of embedded and mounted human brain sections
Corey M Porter1, Sarah Tabler1, Sooin Choi1
1Department of Molecular and Integrative Physiology, University of Michigan, BSRB, 109 Zina Pitcher Place, Ann Arbor, MI, 48109, USA.
Scientific Reports
|June 3, 2024
Summary
Researchers developed TSWIFT, a novel staining method for formalin-fixed, paraffin-embedded brain tissue. This technique enables repeated analysis of mounted brain sections, advancing neurodegenerative disease research.
Area of Science:
- Neuroscience
- Biochemistry
- Pathology
Background:
- Characterizing protein networks in mounted brain tissue is crucial for understanding brain and neurodegenerative diseases.
- Existing methods face challenges in comprehensive protein network analysis.
Purpose of the Study:
- To develop a simple, iterative staining method for mounted brain sections.
- To enable high-dimensional sample phenotyping of formalin-fixed, paraffin-embedded (FFPE) tissues.
Main Methods:
- Developed TSWIFT (Tissue Staining With Iterative Fluorescence Tagging), a novel staining protocol.
- Applied TSWIFT to pre-mounted FFPE human brain sections.
- Assessed tissue architecture conservation and relabeling capability over time.
Main Results:
- TSWIFT successfully preserves tissue architecture.
- A single FFPE brain section can be relabeled more than 10 times, even after extended storage.
- The method allows for iterative, high-dimensional phenotyping of cellular proteomes.
Conclusions:
- TSWIFT is an efficient and robust method for analyzing mounted FFPE human brain tissue.
- This technique facilitates integrated, high-dimensional proteomic knowledge acquisition.
- TSWIFT advances research in brain disorders and neurodegeneration.
