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Updated: May 6, 2026
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
Published on: June 5, 2020
A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
Bernadette Jeremiasse1,2, Ravian L van Ineveld1,2, Veerle Bok1,2
1Princess Máxima Center for Pediatric Oncology, Utrecht, The Netherlands.
Abstract:
Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.
Insights
This study introduces a novel multispectral 3D imaging platform using organoids to rapidly screen molecular probes for fluorescence-guided surgery. This approach models patient tumor heterogeneity, improving probe selection for complete tumor resection.
Area of Science:
- Surgical Oncology
- Biomedical Imaging
- Translational Medicine
Background:
- Complete tumor resection is crucial but challenging.
- Real-time fluorescence-guided surgery (FGS) with molecular probes can improve resection.
- Current pre-clinical probe validation is slow and limited by tumor heterogeneity.
Purpose of the Study:
- To develop a patient-scale platform for rapid screening of multiple molecular probes.
- To model patient tumor heterogeneity and healthy tissue binding using organoids.
- To accelerate the identification of effective probes for FGS.
Main Methods:
- Development of a multispectral real-time 3D imaging platform.
- Implementation of patient-derived tumor organoids to model heterogeneity.
- Utilizing organoids to assess molecular probe binding to both tumor and healthy tissues.
Main Results:
- The platform effectively models patient tumor heterogeneity.
- Organoid technology accurately reflects in vivo probe binding.
- Demonstrated capability for patient-scale probe screening.
Conclusions:
- The developed imaging platform and organoid model offer a powerful tool for pre-clinical probe evaluation.
- This approach accelerates the development of targeted probes for FGS.
- Facilitates improved surgical outcomes through enhanced tumor visualization.
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