Structure-Assisted Boronic Acid Implanted Mesoporous Metal-Organic Frameworks for Specific Extraction of cis-Diol...

Area of Science:

• Materials Science
• Analytical Chemistry
• Biochemistry

Background:

• Cis-diol-containing molecules are vital in biological systems, but their analysis faces challenges due to low abundance and interference.
• Metal-organic frameworks (MOFs) are effective sorbents for sample preparation, yet most are limited by small pores, hindering broader applications.

Purpose of the Study:

• To develop a novel boronate affinity MOF with enhanced porosity for improved cis-diol analysis.
• To overcome the limitations of existing MOFs in analyzing cis-diol-containing compounds.

Main Methods:

• A postsynthetic ligand-exchange strategy was employed to construct EPBA-PCN-333(Fe) MOFs, preserving porosity and crystallinity.
• Click chemistry facilitated the ligand-exchange process, ensuring structural integrity.
• The MOF's performance was evaluated using various cis-diol molecules, including small molecules, glycopeptides, and glycoproteins.

Main Results:

• EPBA-PCN-333(Fe) demonstrated excellent selectivity, efficient diffusion, good accessibility, and a size exclusion effect.
• The binding capacity for catechol was determined to be 0.25 mmol/g with a limit of detection (LOD) of 200 ng/mL.
• The MOF successfully detected nucleosides in human urine samples, showcasing real-world applicability.

Conclusions:

• EPBA-PCN-333(Fe) represents a significant advancement in MOF design for cis-diol analysis.
• The developed MOF offers a promising platform for sensitive and selective detection of cis-diol-containing biomolecules.