Draft genome of a human-derived pks+ E. coli that caused spontaneous disseminated infection in a mouse

Area of Science:

• Microbiology
• Genomics
• Human Microbiome Research

Background:

• Escherichia coli is a common gut bacterium, with most strains being harmless commensals.
• Understanding the factors that allow commensal bacteria to cause extraintestinal infections is crucial for public health.
• The human gut microbiome plays a significant role in host health and disease.

Purpose of the Study:

• To present the draft genome of a novel human-derived Escherichia coli strain.
• To investigate the pathogenic potential of this commensal strain by assessing its ability to disseminate in a host.
• To identify genetic factors that may contribute to extraintestinal dissemination.

Main Methods:

• Isolation and whole-genome sequencing of a novel human-derived Escherichia coli strain.
• In vivo colonization and dissemination studies in a mouse model.
• Bioinformatic analysis of the draft genome to identify virulence factors and genomic features.

Main Results:

• A novel human-derived Escherichia coli strain was successfully isolated from a healthy control subject.
• The draft genome revealed specific genetic characteristics of this strain.
• Upon introduction into a mouse model, the bacterium spontaneously disseminated from the gastrointestinal tract to the kidneys, indicating extraintestinal pathogenic potential.

Conclusions:

• This study highlights the potential for commensal Escherichia coli strains from the human gut microbiome to cause extraintestinal infections.
• The characterized genome provides a foundation for future research into the mechanisms of dissemination and pathogenesis.
• Further investigation is warranted to understand the implications of such strains in human health and disease.