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A Microfluidic Chip-Based Automated System for Whole-Course Monitoring the Drug Responses of Organoids.

Kexin Zhang¹, Jiyu Xi¹, Yadong Wang^2,3

¹Department of Biomedical Engineering, School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.

Analytical Chemistry

|June 4, 2024

Summary

This study introduces a microfluidic chip for real-time adenosine triphosphate (ATP) monitoring in patient-derived organoids, enabling dynamic drug response evaluation for personalized lung cancer therapy.

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Last Updated: Jun 24, 2025

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Published on: April 17, 2021

Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis

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The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Published on: April 28, 2015

Area of Science:

Biomedical Engineering
Oncology
Microfluidics

Background:

Patient-derived organoids are valuable preclinical models for predicting ex vivo drug responses.
Current adenosine triphosphate (ATP) detection methods for organoids are limited to single time points, neglecting dynamic cellular changes.
Limited organoid availability per patient restricts comprehensive time-point drug testing.

Purpose of the Study:

To develop a multifunctional microfluidic chip for comprehensive organoid-based drug response testing.
To enable whole-course monitoring of organoid growth status using a novel ATP sensor.
To establish a clinically applicable automated system for lung cancer drug testing.

Main Methods:

Development of a microfluidic chip integrating organoid establishment, culture, drug treatment, and ATP monitoring.
Integration of a specialized ATP sensor for continuous monitoring of living organoids.
Automation of the entire organoid drug testing process.

Main Results:

The microfluidic chip successfully performed all stages of organoid-based drug response assessment.
The developed ATP sensor enabled the first successful whole-course monitoring of organoid growth.
The automated system demonstrated improved data repeatability and reduced human error compared to conventional methods.

Conclusions:

The microfluidic chip-based automated system provides a more accurate and comprehensive evaluation of drug effects on lung cancer organoids.
This technology bridges the gap between laboratory research and clinical application for personalized medicine.
Continuous ATP monitoring offers deeper insights into dynamic drug responses in organoid models.