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A Microfluidic Chip-Based Automated System for Whole-Course Monitoring the Drug Responses of Organoids

Kexin Zhang1, Jiyu Xi1, Yadong Wang2,3

  • 1Department of Biomedical Engineering, School of Medical Technology, Beijing Institute of Technology, Beijing 100081, China.

Analytical Chemistry
|June 4, 2024
PubMed

Insights

This study introduces a microfluidic chip for real-time adenosine triphosphate (ATP) monitoring in patient-derived organoids, enabling dynamic drug response evaluation for personalized lung cancer therapy.

Area of Science:

  • Biomedical Engineering
  • Oncology
  • Microfluidics

Background:

  • Patient-derived organoids are valuable preclinical models for predicting ex vivo drug responses.
  • Current adenosine triphosphate (ATP) detection methods for organoids are limited to single time points, neglecting dynamic cellular changes.
  • Limited organoid availability per patient restricts comprehensive time-point drug testing.

Purpose of the Study:

  • To develop a multifunctional microfluidic chip for comprehensive organoid-based drug response testing.
  • To enable whole-course monitoring of organoid growth status using a novel ATP sensor.
  • To establish a clinically applicable automated system for lung cancer drug testing.

Main Methods:

  • Development of a microfluidic chip integrating organoid establishment, culture, drug treatment, and ATP monitoring.
  • Integration of a specialized ATP sensor for continuous monitoring of living organoids.
  • Automation of the entire organoid drug testing process.

Main Results:

  • The microfluidic chip successfully performed all stages of organoid-based drug response assessment.
  • The developed ATP sensor enabled the first successful whole-course monitoring of organoid growth.
  • The automated system demonstrated improved data repeatability and reduced human error compared to conventional methods.

Conclusions:

  • The microfluidic chip-based automated system provides a more accurate and comprehensive evaluation of drug effects on lung cancer organoids.
  • This technology bridges the gap between laboratory research and clinical application for personalized medicine.
  • Continuous ATP monitoring offers deeper insights into dynamic drug responses in organoid models.

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