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Temperature-dependent structural changes in xylanase II from Trichoderma longibrachiatum.

Ki Hyun Nam1

  • 1College of General Education, Kookmin University, Seoul, 02707, Republic of Korea.

Carbohydrate Research
|June 4, 2024
PubMed
Summary

Temperature significantly impacts endo-β-1,4-xylanase structure and function. This study reveals distinct conformational changes in xylanase at room versus cryogenic temperatures, crucial for industrial applications.

Keywords:
Conformational changeCrystal structureEndo-β-1,4-xylanasesFlexibilityTemperatureXylanase

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Area of Science:

  • Biochemistry
  • Structural Biology
  • Enzymology

Background:

  • Endo-β-1,4-xylanases are vital enzymes for degrading plant heteroxylans, with broad industrial applications.
  • Temperature is a critical factor influencing xylanase activity, yet its structural effects remain unclear.

Purpose of the Study:

  • To elucidate the structural influence of temperature on xylanase.
  • To compare the crystal structures of xylanase II from Trichoderma longibrachiatum (TloXynII) at room and cryogenic temperatures.

Main Methods:

  • X-ray crystallography was used to determine the structure of TloXynII at 2.1 Å (room temperature) and 1.9 Å (cryogenic temperature).
  • Comparative analysis of B-factor values, domain flexibility, substrate binding cleft, and water molecule networks between the two structures.

Main Results:

  • TloXynII at room temperature (TloXynIIRT) exhibited higher flexibility (B-factor 2.09x) than at cryogenic temperature (TloXynIICryo).
  • Distinct domain flexibility was observed: thumb domain in TloXynIIRT and finger domain in TloXynIICryo.
  • The substrate binding cleft differed in width, with TloXynIIRT being narrower and TloXynIICryo showing extensive water networks.

Conclusions:

  • Temperature induces significant conformational changes in TloXynII, affecting domain flexibility and substrate binding site characteristics.
  • Understanding these temperature-dependent structural dynamics is essential for optimizing xylanase applications in various industries.