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Related Concept Videos

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

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Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
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Oligosaccharide Assembly01:24

Oligosaccharide Assembly

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Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
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Monosaccharide composition analysis by 2D quantitative gsHSQCi.

Xiaofang Ma1, Caihong Li2, Junyin Zhang2

  • 1State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 611137, China; Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, 611137, China.

Carbohydrate Research
|June 4, 2024
PubMed
Summary
This summary is machine-generated.

A novel quantitative 2D NMR method directly measures monosaccharide composition in polysaccharides without derivatization. This technique offers accurate quantification for structural analysis and quality control of polysaccharide preparations.

Keywords:
AnomerFructoseMonosaccharide compositionQuantitative NMRRelaxation enhancement

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Area of Science:

  • Analytical Chemistry
  • Carbohydrate Chemistry
  • Biochemistry

Background:

  • Polysaccharide structure dictates physicochemical properties and biological activities.
  • Monosaccharide composition analysis is crucial for polysaccharide structural characterization and quality control.

Purpose of the Study:

  • To demonstrate a quantitative 2D NMR method for direct monosaccharide quantification.
  • To establish an alternative method for monosaccharide composition analysis in polysaccharides.

Main Methods:

  • Utilized a quantitative 2D NMR method, gsHSQCi (gradient-selective Heteronuclear Single Quantum Coherence), for monosaccharide quantification.
  • Employed a model mixture and tamarind seed polysaccharide for method validation.
  • Investigated Na[Fe(EDTA)] as a relaxation-enhancing agent.

Main Results:

  • The gsHSQCi method accurately quantified various monosaccharides in solution without derivatization or column separation.
  • Achieved precise fructose quantification in a complex nine-monosaccharide mixture, outperforming 1D 1H NMR and PMP-HPLC.
  • Demonstrated the utility of both anomeric and non-anomeric signals for content determination.

Conclusions:

  • The gsHSQCi NMR approach provides a precise and accurate alternative for monosaccharide composition analysis.
  • This method simplifies polysaccharide structural elucidation and quality control.
  • Na[Fe(EDTA)] can be used to reduce experimental time for saccharide analysis.