Inappropriate use of antibiotic enhances antibiotic resistance dissemination in ESBL-EC: Role of ydcz in outer membrane vesicles biogenesis and protein transport
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View abstract on PubMed
Summary
This summary is machine-generated.Inappropriate antibiotic use boosts outer membrane vesicle (OMV) secretion in resistant E. coli, increasing resistance and aiding survival. This highlights YdcZ protein
Area Of Science
- Microbiology
- Molecular Biology
- Public Health
Background
- Extended-spectrum beta-lactamase producing Escherichia coli (ESBL-EC) in poultry is a public health concern.
- Antibiotic resistance in ESBL-EC poses significant threats.
- Inappropriate antibiotic use is a suspected driver of resistance.
Purpose Of The Study
- Investigate how inappropriate antibiotic use affects ESBL-EC antibiotic resistance.
- Explore molecular mechanisms, particularly vesicle formation and protein secretion.
- Hypothesize that amoxicillin exacerbates resistance via OMV changes.
Main Methods
- Experimental analysis of ESBL-EC exposed to amoxicillin.
- Quantification of secreted proteins in outer membrane vesicles (OMVs).
- Proteomic analysis and gene knockout experiments (YdcZ).
- Assessing OMV protection against sensitive bacteria.
Main Results
- Amoxicillin increased OMV protein concentration and CTX-M-55 beta-lactamase expression in ESBL-EC.
- YdcZ protein identified as key in OMV formation and amoxicillin-induced protein transport.
- YdcZ knockout reduced OMV protein content and protection against sensitive bacteria.
Conclusions
- YdcZ plays a critical role in amoxicillin-induced OMV protein secretion in ESBL-EC.
- Inappropriate antibiotic use promotes OMV secretion, aiding resistant bacteria survival.
- Findings offer insights into antibiotic resistance dissemination mechanisms.
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