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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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Assembly of the Tn7 targeting complex by a regulated stepwise process.

Yao Shen¹, Shreya S Krishnan¹, Michael T Petassi²

¹Department of Biochemistry, McGill University, Montreal, QC H3G 0B1, Canada; Centre de recherche en biologie structurale (CRBS), McGill University, Montreal, QC H3G 0B1, Canada.

|June 4, 2024

Summary

The Tn7 transposon system uses TnsC and TniQ proteins to precisely insert DNA. Structural studies reveal how ATP binding to TnsC controls its assembly into a ring, ensuring accurate transposition.

Keywords:

AAA+ ATPase DNA transposition Tn7 transposon TnsD/TniQ proteins cryo-EM nucleotide-exchange factor protein-DNA interactions target-site selection transposition immunity

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Area of Science:

Molecular Biology
Structural Biology
Genetics

Background:

The Tn7 family of transposons exhibits highly regulated transposition.
Targeting involves TniQ proteins and the TnsC adaptor to recruit transposase.
Understanding the mechanism of target selection and DNA integration is crucial.

Purpose of the Study:

To elucidate the structural basis of TnsC regulation by TniQ during Tn7 transposition.
To understand the role of ATP/ADP binding in TnsC assembly and function.
To provide insights into the precise and oriented DNA insertion mechanism of Tn7.

Main Methods:

Cryoelectron microscopy (cryo-EM) to determine the structures of TnsC bound to the TniQ domain of TnsD.
Biochemical assays to study the nucleotide-dependent behavior of TnsC.

Main Results:

Determined cryo-EM structures of TnsC complexed with TniQ domain of TnsD.
Revealed distinct conformational states of TnsC based on ATP versus ADP binding.
Showed TnsD acts as an exchange factor, facilitating unidirectional loading of ATP-bound TnsC into a heptameric ring.
Identified functionally distinct TnsC protomers within the assembled ring.

Conclusions:

The TnsD-TnsC interaction and nucleotide exchange mechanism ensure unidirectional TnsC ring assembly at target sites.
Functionally distinct TnsC protomers regulate target immunity and ensure precise, oriented DNA insertion by Tn7 transposons.