Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

578
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
578

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Wavelength Rule for the Analysis of Clusteroluminescence.

Polymers·2025
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.1K

A simple light path modifying device to reduce scattering in front-face fluorescence spectra.

Frank B Peters1, Andreas O Rapp1

  • 1Institut für Berufswissenschaften im Bauwesen, Leibniz-Universität Hannover, Herrenhäuser Straße 8, 30419 Hannover, Germany.

Methods and Applications in Fluorescence
|June 4, 2024
PubMed
Summary

A new light path modifying (LPM) device reduces scattering signals in front-face fluorescence spectroscopy. This innovation improves spectral clarity and avoids peak distortion, enhancing fluorescence measurements for various substances.

Keywords:
devicefluorescencefront-facescatteringsolid sample

More Related Videos

Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

27.9K
Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

17.6K

Related Experiment Videos

Last Updated: Jun 24, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.1K
Video-rate Scanning Confocal Microscopy and Microendoscopy
14:10

Video-rate Scanning Confocal Microscopy and Microendoscopy

Published on: October 20, 2011

27.9K
Lensless Fluorescent Microscopy on a Chip
11:23

Lensless Fluorescent Microscopy on a Chip

Published on: August 17, 2011

17.6K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Optics

Background:

  • Front-face fluorescence spectroscopy is crucial for analyzing various materials.
  • Scattering signals can interfere with accurate fluorescence measurements, distorting spectral data.
  • Minimizing scattering is essential for obtaining clear and reliable fluorescence spectra.

Purpose of the Study:

  • To present a novel device for diminishing scattering signals in front-face fluorescence spectra.
  • To evaluate the effectiveness of the light path modifying (LPM) device in improving the scattering-to-fluorescence ratio.
  • To demonstrate the impact of the LPM device on spectral clarity and peak fidelity.

Main Methods:

  • Modification of a commercial fluorescence spectrometer's beam path using two deflecting mirrors.
  • Implementation of a light path modifying (LPM) device to direct reflections away from the sensor.
  • Testing the LPM device with two fluid and three solid substances.

Main Results:

  • The scattering-to-fluorescence ratio improved significantly, ranging from 1.7 to 7.6.
  • Spectra obtained using the LPM device were markedly clearer compared to unmodified spectra.
  • Distortion of fluorescence peaks was effectively avoided, ensuring spectral integrity.

Conclusions:

  • The developed LPM device successfully reduces scattering interference in front-face fluorescence spectroscopy.
  • The device enhances spectral quality, leading to more accurate fluorescence analysis.
  • The LPM device offers a practical solution for improving fluorescence measurements across diverse sample types.