Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Ythdf1's Grip On Crc Vasculature: Insights Into Linc01106 And Mir-449b-5p-vegfa Axis.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Ythdf1's Grip On Crc Vasculature: Insights Into Linc01106 And Mir-449b-5p-vegfa Axis.

Related Experiment Video

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

8.1K

YTHDF1's grip on CRC vasculature: insights into LINC01106 and miR-449b-5p-VEGFA axis.

Rui-Ting Ma1,2, Yuanyuan Wang1, Feng Ji1

  • 1Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, Jiangsu, 210023, China.

Cancer Cell International
|June 4, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

This study reveals that YTHDF1 targets LINC01106 for m6A modification, suppressing colorectal cancer (CRC) vascular generation. This finding offers new insights into CRC progression and potential therapeutic strategies.

Keywords:
Colorectal cancerLinc01106Mir-449b-5pVascular generation

More Related Videos

A Method for Labeling Vasculature in Embryonic Mice
09:58

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

15.7K
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

11.3K

Related Experiment Videos

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice
08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

8.1K
A Method for Labeling Vasculature in Embryonic Mice
09:58

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

15.7K
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
07:49

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

11.3K

Area of Science:

  • Molecular Biology
  • Oncology
  • RNA Biology

Background:

  • Investigating long noncoding RNA (lncRNA) N6-methyladenosine (m6A) modification in colorectal cancer (CRC) vasculature.
  • Focusing on the roles of LINC01106 and YTHDF1 in CRC progression.

Purpose of the Study:

  • To elucidate the mechanism of LINC01106 and YTHDF1 in CRC vascular generation.
  • To determine the prognostic significance of YTHDF1 in CRC.

Main Methods:

  • Clinical assessments of LINC01106 expression and its correlation with vascular generation.
  • Functional experiments to evaluate YTHDF1's impact on CRC cell dynamics.
  • Methylated RNA immunoprecipitation (Me-RIP) and individual-nucleotide-resolution crosslinking immunoprecipitation (Me-CLIP) assays to identify m6A modification and target interactions.
Ythdf1
m6a modification

Main Results:

  • Upregulated LINC01106 promotes vascular generation through the miR-449b-5p-VEGFA pathway.
  • Elevated YTHDF1 expression in CRC tissues is associated with poor prognosis.
  • YTHDF1 targets m6A-modified LINC01106, inhibiting CRC cell proliferation and angiogenesis.

Conclusions:

  • YTHDF1-mediated m6A modification of LINC01106 plays a crucial role in suppressing colorectal cancer vascular generation.
  • This regulatory axis represents a potential therapeutic target for CRC treatment.