Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Legal Framework and Practical Challenges of Secondary Use of Medical Data in Japan.

Studies in health technology and informatics·2026

Same author

Less Invasive Light Irradiation Methods for Inner Ear Gene Therapy Based on Photoswitch Technology: Toward Clinical Translation.

Human gene therapy·2026

Same author

A chemically inducible, leakless Cre recombinase by split-protein-based efficient and enhanced degradation (SPEED).

Communications biology·2025

Same author

AlphaFold3-guided optimization of a photoactivatable endonuclease for top-down genome engineering.

The Journal of biological chemistry·2025

Same author

Impact of family physician-led hospitalist intervention methods in the multidisciplinary management of hip fracture: A retrospective cohort study.

Australian journal of general practice·2025

Same author

An Examination of Japan's Legal System and Issues Related to Promoting the Use of Medical Data.

Studies in health technology and informatics·2025

Search research articles

Related Experiment Video

Updated: Jun 24, 2025

Imaging Protein-protein Interactions in vivo

Imaging Protein-protein Interactions in vivo

Published on: October 10, 2010

Highly Efficient Fluorescent Probe to Visualize Protein Interactions at the Superresolution.

Yuki Aono¹, Takahiro Nakajima^1,2, Wataru Ichimiya¹

¹Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan.

ACS Chemical Biology

|June 5, 2024

Summary

This summary is machine-generated.

Researchers developed split-Dendra2, a new fluorescent probe for superresolution microscopy. This tool efficiently visualizes protein-protein interactions in cells, enabling nanoscale insights into cellular functions.

More Related Videos

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

Related Experiment Videos

Last Updated: Jun 24, 2025

Imaging Protein-protein Interactions in vivo

Imaging Protein-protein Interactions in vivo

Published on: October 10, 2010

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Published on: March 14, 2019

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation BiFC-PALM

Published on: December 22, 2015

Area of Science:

Cellular Biology
Microscopy
Biochemistry

Background:

Superresolution microscopy (SR microscopy) is crucial for studying protein-protein interactions (PPIs) within subcellular structures.
Existing SR microscopy technologies for PPIs lack sufficient power and utility.
Understanding PPIs at the nanoscale is vital for elucidating cellular functions.

Purpose of the Study:

To develop a novel, highly efficient fluorescent probe for SR microscopy of PPIs.
To enable high-resolution visualization of PPIs in small subcellular compartments.
To overcome limitations of current tools for SR microscopy of PPIs.

Main Methods:

Development of a split-fluorescent protein probe, split-Dendra2.
Application of split-Dendra2 for SR microscopy of cellular samples.
Demonstration of PPI visualization in specific subcellular structures like clathrin-coated pits and focal adhesions.

Main Results:

Split-Dendra2 demonstrated highly efficient detection of PPIs.
Achieved SR microscopy of PPIs with high spatial resolution and image reconstruction fidelity.
Successfully visualized previously unresolvable PPIs in small subcellular structures.

Conclusions:

Split-Dendra2 is a powerful and useful tool for SR microscopy of PPIs.
The probe significantly expands the capabilities of SR microscopy.
Facilitates the study of PPI functions at the nanoscale resolution.