Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

H C Birnboim, J Doly

    Nucleic Acids Research
    |November 24, 1979
    PubMed
    Summary

    This study presents a simple plasmid DNA extraction method for bacterial cells. The technique allows for high-throughput analysis and yields pure plasmid DNA suitable for restriction enzyme digestion.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A method for assessing efficiency of bacterial cell disruption and DNA release.

    BMC microbiology·2016
    Same author

    Expression of thymidylate synthase in human cells is an early G(1) event regulated by CDK4 and p16INK4A but not E2F.

    British journal of cancer·2007
    Same author

    Nitric oxide donors induce large-scale deletion mutations in human lymphoblastoid cells: implications for mutations in T-lymphocytes from arthritis patients.

    Environmental and molecular mutagenesis·2002
    Same author

    Inhibition of histone deacetylation induces constitutive derepression of the beta interferon promoter and confers antiviral activity.

    Journal of virology·2001
    Same author

    Expression of interleukin-8 promotes neutrophil infiltration and genetic instability in mutatect tumors.

    Neoplasia (New York, N.Y.)·2001
    Same author

    Constitutive expression of interleukin-8 by Mutatect cells markedly affects their tumor biology.

    Carcinogenesis·2001

    Area of Science:

    • Molecular Biology
    • Biochemistry

    Background:

    • Plasmid DNA extraction is crucial for molecular biology techniques.
    • Existing methods can be time-consuming or yield DNA of insufficient purity.

    Purpose of the Study:

    • To describe a simple and efficient method for plasmid DNA extraction from bacterial cells.
    • To enable high-throughput analysis of bacterial clones.

    Main Methods:

    • Selective alkaline denaturation of chromosomal DNA.
    • pH control without a pH meter.
    • Neutralization to precipitate chromosomal DNA, leaving plasmid DNA in solution.

    Main Results:

    • High-throughput analysis of over 100 clones per day is possible.
    • Extracted plasmid DNA is pure enough for restriction enzyme digestion.
    • The method successfully extracts both large and small plasmid DNAs.

    Conclusions:

    • This method offers a simple, rapid, and effective way to isolate plasmid DNA.
    • The extracted plasmid DNA is suitable for downstream molecular applications.
    • The procedure is adaptable for various plasmid sizes.

    Related Experiment Videos