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m6A modification of lncRNA ABHD11-AS1 promotes colorectal cancer progression and inhibits ferroptosis through TRIM21/IGF2BP2/ FOXM1 positive feedback loop

  • 0Department of Oncology, Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China; State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of digestive Disease, Fourth Military Medical University, Xi'an, 710032, China.
Cancer Letters +

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June 5, 2024

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June 5, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Long non-coding RNA ABHD11-AS1 is highly expressed in colorectal cancer (CRC), suppressing ferroptosis and promoting tumor growth. This lncRNA may serve as a novel therapeutic target for CRC treatment.

Area Of Science

  • Molecular Biology
  • Oncology
  • Genetics

Background

  • Long non-coding RNAs (lncRNAs) are implicated in various human cancers, offering potential as diagnostic and therapeutic biomarkers.
  • The specific roles and mechanisms of most lncRNAs in colorectal cancer (CRC) remain largely unknown.

Purpose Of The Study

  • To investigate the clinical significance and underlying mechanisms of lncRNA ABHD11 antisense RNA 1 (ABHD11-AS1) in colorectal cancer.
  • To explore the potential of ABHD11-AS1 as a therapeutic target for CRC.

Main Methods

  • Quantitative real-time PCR to assess ABHD11-AS1 expression in CRC patients.
  • In vitro and in vivo experiments to evaluate the functional roles of ABHD11-AS1 in CRC proliferation, migration, and ferroptosis.
  • RNA immunoprecipitation and Western blotting to elucidate the molecular interactions involving ABHD11-AS1, IGF2BP2, FOXM1, TRIM21, and METTL3.

Main Results

  • ABHD11-AS1 expression was significantly upregulated in CRC tissues and correlated with poor prognosis.
  • ABHD11-AS1 inhibited ferroptosis and promoted CRC cell proliferation and migration.
  • ABHD11-AS1 stabilized FOXM1 by interacting with IGF2BP2, forming a positive feedback loop.
  • TRIM21 mediated IGF2BP2 degradation, and ABHD11-AS1 facilitated this interaction.
  • METTL3 enhanced ABHD11-AS1 stability via the m6A reader IGF2BP2.

Conclusions

  • lncRNA ABHD11-AS1 acts as a crucial oncogenic regulator in colorectal cancer.
  • ABHD11-AS1 exerts its functions by modulating ferroptosis, proliferation, and migration through the ABHD11-AS1/IGF2BP2/FOXM1 axis.
  • ABHD11-AS1 represents a promising therapeutic target for colorectal cancer treatment.

Keywords:

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