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Updated: May 4, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Optimising parameters for pilot scale steam explosion and continuous pressurised disc refining of Miscanthus and
David Warren-Walker1, Sreenivas Rao Ravella1, Joe Gallagher1
1Institute of Biological Environmental and Rural Sciences, Aberystwyth University, Aberystwyth SY23 3EB, UK.
Abstract:
The first comparative pre-treatment study of Miscanthus (Mxg) and sugarcane bagasse (SCB) using steam explosion (SE) and pressurised disc refining (PDR) pretreatment to optimise xylose and xylo-oligosaccharide release is described. The current investigation aimed to 1) Develop optimised batch-wise steam explosion parameters for Mxg and SCB, 2) Scale from static batch steam explosion to dynamic continuous pressurised disc refining, 3) Identify, understand, and circumvent scale-up production hurdles. Optimised SE parameters released 82% (Mxg) and 100% (SCB) of the available xylan. Scaling to PDR, Miscanthus yielded 85% xylan, highlighting how robust scouting assessments for boundary process parameters can result in successful technical transfer. In contrast, SCB technical transfer was not straightforward, with significant differences observed between the two processes, 100% (SE) and 58% (PDR). This report underlines the importance of feedstock-specific pretreatment strategies to underpin process development, scale-up, and optimisation of carbohydrate release from biomass.
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