Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The structural basis for the phospholipid remodeling by lysophosphatidylcholine acyltransferase 3.

Nature communications·2021
Same author

Cytotoxic new caged-polyprenylated xanthonoids from Garcinia oligantha.

Fitoterapia·2021
Same author

Chlorophyll fluorescence as a light signal enhances iron uptake by the marine diatom Phaeodactylum tricornutum under high-cell density conditions.

BMC biology·2021
Same author

Accelerating the peroxidase-like activity of Co<sup>2+</sup> by quinaldic acid: Mechanism and its analytical applications.

Talanta·2021
Same author

Collision of two self-trapped atomic matter wave packets in an optical ring cavity.

Physical review. E·2021
Same author

Performance of Plasma Amyloid β, Total Tau, and Neurofilament Light Chain in the Identification of Probable Alzheimer's Disease in South China.

Frontiers in aging neuroscience·2021
Same journal

Optimization of mode discerning control for nonlinear hybrid systems subject to unknown inputs with applications to active fault diagnosis.

ISA transactions·2026
Same journal

Convergence evaluation of optimization-based stochastic iterative learning control.

ISA transactions·2026
Same journal

Adaptive utility-aware event-triggered reinforcement learning for hybrid attack scheduling against remote state estimation.

ISA transactions·2026
Same journal

Hybrid vehicle state estimation using closed-form liquid neural networks and nonlinear Kalman filtering.

ISA transactions·2026
Same journal

Cross-coupled synchronization control strategy for rebar binding robots based on impedance control.

ISA transactions·2026
Same journal

Gas flow tracking for electronic pressure control system in gas chromatography under state constraints and hysteresis：An innovative fuzzy adaptive control approach.

ISA transactions·2026
See all related articles

Related Experiment Video

Updated: Jun 24, 2025

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish
14:03

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

11.0K

Zebrafishtracker3D: A 3D skeleton tracking algorithm for multiple zebrafish based on particle matching.

Zhenhua Fu1, Shuhui Zhang2, Lu Zhou1

  • 1Institute of Robotics and Automatic Information System (IRAIS) and Tianjin Key Laboratory of Intelligent Robotic (tjKLIR), Nankai University, Tianjin, 300071, China; Institute of Intelligence Technology and Robotic Systems, Shenzhen Research Institute of Nankai University, Shenzhen, 518063, China.

ISA Transactions
|June 5, 2024
PubMed
Summary
This summary is machine-generated.

Zebrafishtracker3D accurately tracks multiple zebrafish in 3D, even with occlusion. This novel algorithm provides detailed skeleton data for advanced behavioral analysis in biological research.

Keywords:
Behavioral analysisImage morphologyMulti-object trackingStereo matchingZebrafish

More Related Videos

Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish
09:40

Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish

Published on: October 31, 2016

12.9K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K

Related Experiment Videos

Last Updated: Jun 24, 2025

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish
14:03

Using an Automated 3D-tracking System to Record Individual and Shoals of Adult Zebrafish

Published on: December 5, 2013

11.0K
Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish
09:40

Using Touch-evoked Response and Locomotion Assays to Assess Muscle Performance and Function in Zebrafish

Published on: October 31, 2016

12.9K
A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K

Area of Science:

  • * Biological Sciences
  • * Biomedical Research
  • * Ethology

Background:

  • * Zebrafish are valuable model organisms due to high human gene homology.
  • * Efficient behavioral analysis requires advanced visual tracking.
  • * Existing methods struggle with multi-zebrafish occlusion and lack detailed data.

Purpose of the Study:

  • * To develop an algorithm for robust 3D multi-zebrafish tracking.
  • * To overcome occlusion challenges in vision-based tracking.
  • * To enable detailed behavioral analysis through skeleton estimation.

Main Methods:

  • * Zebrafishtracker3D utilizes a skeleton stability strategy to reduce errors from zebrafish overlap.
  • * Estimates zebrafish skeletons using head coordinates from top-view.
  • * Employs a particle-matching method for 3D tracking by optimizing front- and top-view matching.

Main Results:

  • * Achieved >90% multiple object tracking accuracy (MOTA) in top-view.
  • * Demonstrated >90% 3D tracking matching accuracy in complex, overlapping scenarios.
  • * Successfully applied to zebrafish courtship experiments for behavioral analysis.

Conclusions:

  • * Zebrafishtracker3D is the first algorithm for simultaneous 3D skeleton tracking of multiple zebrafish.
  • * The method is robust to occlusion and suitable for life science applications.
  • * Provides detailed skeleton data crucial for in-depth zebrafish behavior research.