A fluorescent probe based on cyclochalcone for detecting peroxynitrite

Area of Science:

• Analytical Chemistry
• Biochemistry
• Materials Science

Background:

• Peroxynitrite (ONOO-) is a reactive nitrogen species implicated in various physiological and pathological processes.
• Sensitive and selective detection methods for ONOO- are crucial for biological and medical research.
• Fluorescent probes offer advantages in sensitivity and real-time monitoring for reactive species detection.

Purpose of the Study:

• To synthesize and characterize a novel cyclic chalcone fluorescent probe, C-PN.
• To evaluate the probe's performance for the detection of peroxynitrite (ONOO-).
• To investigate the mechanism of fluorescence change upon reaction with ONOO-.

Main Methods:

• Synthesis of the cyclic chalcone fluorescent probe C-PN.
• Spectroscopic analysis (fluorescence and UV-Vis) to study the probe's response to ONOO-.
• Evaluation of probe selectivity, stability, response time, and detection limit.

Main Results:

• The synthesized probe C-PN exhibited a distinct color change and fluorescence shift upon reaction with ONOO-.
• The probe demonstrated a short response time (<60 s) and good selectivity and stability for ONOO-.
• A low detection limit of 20.2 nM was achieved, enabling sensitive detection of ONOO-.

Conclusions:

• The novel cyclic chalcone fluorescent probe C-PN is effective for the naked-eye and fluorescence detection of ONOO-.
• The probe's mechanism involves the disconnection of the double bond in the cyclic chalcone structure, altering intramolecular charge transfer.
• C-PN shows promise as a reliable tool for ONOO- sensing in relevant chemical and biological contexts.