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A fluorescent probe based on cyclochalcone for detecting peroxynitrite.

Li-Hao Liu¹, Xian-Zhao Shang¹, Jian-Hao Yuan¹

¹Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.

Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology

|June 5, 2024

Summary

A new fluorescent probe, C-PN, detects peroxynitrite (ONOO-) by changing color and fluorescence. This probe offers rapid, selective, and stable detection of ONOO- for potential applications.

Keywords:

Chalcone Fluorescent probe Peroxynitrite

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Area of Science:

Analytical Chemistry
Biochemistry
Materials Science

Background:

Peroxynitrite (ONOO-) is a reactive nitrogen species implicated in various physiological and pathological processes.
Sensitive and selective detection methods for ONOO- are crucial for biological and medical research.
Fluorescent probes offer advantages in sensitivity and real-time monitoring for reactive species detection.

Purpose of the Study:

To synthesize and characterize a novel cyclic chalcone fluorescent probe, C-PN.
To evaluate the probe's performance for the detection of peroxynitrite (ONOO-).
To investigate the mechanism of fluorescence change upon reaction with ONOO-.

Main Methods:

Synthesis of the cyclic chalcone fluorescent probe C-PN.
Spectroscopic analysis (fluorescence and UV-Vis) to study the probe's response to ONOO-.
Evaluation of probe selectivity, stability, response time, and detection limit.

Main Results:

The synthesized probe C-PN exhibited a distinct color change and fluorescence shift upon reaction with ONOO-.
The probe demonstrated a short response time (<60 s) and good selectivity and stability for ONOO-.
A low detection limit of 20.2 nM was achieved, enabling sensitive detection of ONOO-.

Conclusions:

The novel cyclic chalcone fluorescent probe C-PN is effective for the naked-eye and fluorescence detection of ONOO-.
The probe's mechanism involves the disconnection of the double bond in the cyclic chalcone structure, altering intramolecular charge transfer.
C-PN shows promise as a reliable tool for ONOO- sensing in relevant chemical and biological contexts.