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Updated: Jun 24, 2025
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Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
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A Transcriptomic Dataset of Embryonic Murine Telencephalon
Shohei Ochi1, Shyu Manabe1, Takako Kikkawa1
1Department of Developmental Neuroscience, Tohoku University Graduate School of Medicine, Sendai, 980-8575, Japan.
Scientific Data
|June 5, 2024
Summary
This study reveals significant sex differences in mouse embryonic brain gene expression, particularly during peak neurogenesis at embryonic day 14.5. These findings highlight the importance of the embryonic period for understanding sex-based brain development.
Area of Science:
- Neuroscience
- Developmental Biology
- Genetics
Background:
- Sex biases are observed in neurodevelopmental disorders.
- Perinatal sex hormone exposure influences brain development, but embryonic gene expression differences are less understood.
Purpose of the Study:
- To investigate sex differences in gene expression and cortical organization during mouse embryonic development.
- To analyze telencephalon gene expression at embryonic day 11.5 and 14.5.
Main Methods:
- Comprehensive bulk RNA-sequencing of mouse telencephalon.
- Analysis at embryonic day 11.5 (neural stem cell expansion peak) and embryonic day 14.5 (neurogenesis peak).
Main Results:
- Notable sex differences in gene expression were identified, becoming clear at embryonic day 14.5.
- Differences were less apparent at embryonic day 11.5.
Conclusions:
- Embryonic gene expression patterns exhibit sex-specific divergence during peak neurogenesis.
- Understanding embryonic sex differences is crucial for comprehending sex biases in brain development and neurodevelopmental disorders.
