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Association of the SHOX2 and RASSF1A methylation levels with the pathological evolution of early-stage lung

Jiaping Zhao1,2, Yu Lu1,2, Xiaosha Ren3

  • 1Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong University, No.20 XISI road, ChongChuan District, NanTong, 226001, Jiangsu, China.

BMC Cancer
|June 5, 2024
PubMed
Summary
This summary is machine-generated.

Measuring SHOX2 and RASSF1A methylation levels aids in early lung adenocarcinoma detection and predicting tumor invasiveness. This biomarker offers improved pathological diagnosis before surgery.

Keywords:
RASSF1ASHOX2InvasivenessLung adenocarcinomaMethylation

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Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Biomarker Discovery

Background:

  • Morphological diagnosis of lung cancer has limitations.
  • SHOX2 and RASSF1A methylation show potential as early lung cancer biomarkers.
  • Developing accurate diagnostic tools is crucial for effective lung cancer treatment.

Purpose of the Study:

  • To diagnose lung adenocarcinoma using SHOX2 and RASSF1A methylation levels.
  • To predict lung cancer invasiveness prior to surgery.
  • To provide accurate pathological diagnosis for surgical guidance.

Main Methods:

  • Quantified SHOX2 and RASSF1A methylation using a LungMe® test kit via methylation-specific PCR (MS-PCR).
  • Validated diagnostic efficacy and cutoff values with ROC curve analysis.
  • Calculated hazardous factors for lung adenocarcinoma invasiveness using multiple regression.

Main Results:

  • Established cutoff values for SHOX2 (8.3) and RASSF1A (12.0).
  • LungMe® demonstrated sensitivities of 71.3% for IA, 41.7% for MIA, and 16.1% for AIS patients at 94.1% specificity for benign lesions.
  • SHOX2, RASSF1A, and LungMe® methylation levels correlated with invasiveness indicators like age, tumor size, TNM stage, and pleural invasion.

Conclusions:

  • Combined SHOX2 and RASSF1A methylation serves as an early indicator for lung adenocarcinoma.
  • LungMe® methylation is significantly correlated with various clinicopathological features.
  • SHOX2/RASSF1A methylation levels, tumor size, and CTR values can predict tumor invasiveness, guiding surgical procedures.