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Synthetic insulin-like growth factor II.
Biochemical and Biophysical Research Communications
|March 15, 1985
Summary
Solid-phase synthesis successfully created human insulin-like growth factor II (IGF-II), a 67-amino acid protein. The synthetic IGF-II is biologically identical to the natural hormone, confirmed by various assays.
Area of Science:
- Biochemistry
- Protein synthesis
- Endocrinology
Background:
- Human insulin-like growth factor II (IGF-II) plays a crucial role in growth and development.
- Understanding IGF-II structure and function is vital for therapeutic applications.
- Previous methods for obtaining IGF-II were limited.
Purpose of the Study:
- To synthesize human insulin-like growth factor II (IGF-II) using the solid-phase method.
- To verify the homogeneity and identity of the synthesized IGF-II.
- To compare the biological activity of synthetic IGF-II with natural IGF-II.
Main Methods:
- Solid-phase synthesis of a 67-amino acid peptide.
- Purification and homogeneity assessment using chromatofocusing and high-performance liquid chromatography (HPLC).
- Amino acid analysis for composition verification.
- Radioimmunoassay (RIA) and radioreceptor assay (RRA) for biological activity comparison.
Main Results:
- Successfully synthesized human IGF-II with 67 amino acid residues and three disulfide bridges.
- Homogeneity confirmed by chromatofocusing, HPLC, and amino acid analysis.
- Synthetic IGF-II demonstrated indistinguishable performance from natural IGF-II in RIA and RRA.
Conclusions:
- The solid-phase method is effective for synthesizing human IGF-II.
- Synthetic human IGF-II possesses identical biological activity to the natural hormone.
- This synthetic IGF-II is a viable alternative for research and potential therapeutic use.