Expression of GPR56 reflects a hypoactivated state of circulating B cells and is downregulated in B cell subsets in patients with early-stage lung adenocarcinoma

Ayibaota Bahabayi ¹, Zhao Guan ¹, Mohan Zheng ^1,2

⁰Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.

Immunology +

|

June 6, 2024

View abstract on PubMed

Summary

GPR56 levels in B cells may aid early lung adenocarcinoma (LUAD) detection. Decreased GPR56 in B cells suggests active immunity, potentially serving as a diagnostic marker for LUAD.

Area Of Science

Immunology
Oncology
Cell Biology

Background

Lung adenocarcinoma (LUAD) is a prevalent cancer where early detection is critical.
G protein-coupled receptor 56 (GPR56) is known for its role in T cells, but its function in B cells is unexplored.
Investigating novel biomarkers for LUAD diagnosis is essential.

Purpose Of The Study

To investigate the role and significance of GPR56 in the context of LUAD.
To explore GPR56 expression patterns in B cell subsets in LUAD patients.
To assess the potential of GPR56 as an early diagnostic marker for LUAD.

Main Methods

Flow cytometry analysis of GPR56 expression in circulating B cell subsets.
Assessment of GPR56 expression in B cells following lipopolysaccharide (LPS) stimulation.
Correlation analysis between GPR56+ B cell subsets and established LUAD tumor markers.

Main Results

GPR56 showed increased expression in plasmablasts and decreased expression in new memory B cells.
LPS stimulation led to reduced GPR56 expression and decreased HLA-DR+ and CD40+ proportions in GPR56+ B cells.
Early-stage LUAD patients exhibited significant down-regulation of GPR56 in circulating B cell subsets, correlating with tumor markers.

Conclusions

GPR56 expression in B cells may indicate a hypoactivation state.
A decreased proportion of GPR56+ B cells in LUAD patients suggests active humoral immunity.
GPR56 in B cells holds potential as a valuable biomarker for the early diagnosis of LUAD.

Keywords:

B lymphocyte GPR56 early diagnosis lung adenocarcinoma