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Related Experiment Video

Updated: Jun 24, 2025

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Published on: November 1, 2024

PLASMODESMATA-LOCATED PROTEIN 6 regulates plasmodesmal function in Arabidopsis vasculature.

Zhongpeng Li¹, Su-Ling Liu¹, Christian Montes-Serey²

¹Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, IA 50011, USA.

|June 6, 2024

Summary

Plant cells use plasmodesmata for communication. PLASMODESMATA-LOCATED PROTEINS (PDLPs) regulate callose at plasmodesmata. PDLP6 interacts with SUCROSE SYNTHASE 6 and CALLOSE SYNTHASE 7 in the vasculature to control plasmodesmal function.

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Last Updated: Jun 24, 2025

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Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Identification of Plasmodesmal Localization Sequences in Proteins In Planta

Published on: August 15, 2017

Area of Science:

Plant Biology
Cell Biology
Molecular Biology

Background:

Plasmodesmata are crucial for intercellular transport in plants.
Callose deposition at plasmodesmata regulates their aperture and function.
PLASMODESMATA-LOCATED PROTEINS (PDLPs) are involved in maintaining callose homeostasis.

Purpose of the Study:

To investigate the distinct functions of PDLP5 and PDLP6 in different plant cell types.
To identify functional partners of PDLP5 and PDLP6.
To elucidate the molecular mechanism by which PDLP6 regulates plasmodesmal function.

Main Methods:

Overexpression of PDLP5 and PDLP6 in Arabidopsis thaliana.
Analysis of plasmodesmal callose accumulation and starch patterns.
Enzyme-catalyzed proximity labeling to identify protein interactors.
Co-immunoprecipitation and genetic interaction studies.

Main Results:

PDLP5 and PDLP6 are expressed in non-overlapping cell types and affect callose accumulation differently.
Distinct starch accumulation patterns were observed in PDLP5 and PDLP6 overexpressors.
SUCROSE SYNTHASE 6 (SUS6) was identified as a functional partner of PDLP6 in the vasculature.
PDLP6 physically and genetically interacts with SUS6 and CALLOSE SYNTHASE 7 (CALS7).
CALS7 is essential for PDLP6 function.

Conclusions:

PDLP5 and PDLP6 play distinct roles in regulating plasmodesmal callose in different cell types.
PDLP6, in conjunction with SUS6 and CALS7, functions within the vasculature to modulate plasmodesmal activity.
This study provides insights into the complex regulation of plasmodesmal transport by PDLP proteins and their interacting partners.